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- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pEMBL8-
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pEMBL8-
其他名称:
质粒大小（bp）: 3939
抗性: Ampicillin
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: X04996
其他属性: According to the cloning strategy, the phage f1 origin should be flanked by regenerated EcoRI sites, but these are not found experimentally. The given sequence must be incorrect around this position. The base n was introduced to destroy the EcoRI site (GAAnTTC). ssDNA-producing plasmid with polylinker in lacZ'. Medium is 1227 LB plus ampicillin. Hosts: E.coli 71/18, E.coli, E.coli JM series. Related vectors: pUC8, M13mp8, pEMBL8+.
质粒图谱:
质粒序列: tr
| 1 | gcccaatacg | caaaccgcct | ctccccgcgc | gttggccgat | tcattaatgc | 50 |
| 51 | agctggcacg | acaggtttcc | cgactggaaa | gcgggcagtg | agcgcaacgc | 100 |
| 101 | aattaatgtg | agttagctca | ctcattaggc | accccaggct | ttacacttta | 150 |
| 151 | tgcttccggc | tcgtatgttg | tgtggaattg | tgagcggata | acaatttcac | 200 |
| 201 | acaggaaaca | gctatgacca | tgattacgaa | ttcccgggga | tccgtcgacc | 250 |
| 251 | tgcagccaag | cttggcactg | gccgtcgttt | tacaacgtcg | tgactgggaa | 300 |
| 301 | aaccctggcg | ttacccaact | taatcgcctt | gcagcacatc | cccctttcgc | 350 |
| 351 | cagctggcgt | aatagcgaag | aggcccgcac | cgatcgccct | tcccaacagt | 400 |
| 401 | tgcgcagcct | gaatggcgaa | tggaanttcc | aacatccaat | aaatcataca | 450 |
| 451 | ggcaaggcaa | agaattagca | aaattaagca | ataaagcctc | agagcataaa | 500 |
| 501 | gctaaatcgg | ttgtaccaaa | aacattatga | ccctgtaata | cttttgcggg | 550 |
| 551 | agaagccttt | atttcaacgc | aaggataaaa | atttttagaa | ccctcatata | 600 |
| 601 | ttttaaatgc | aatgcctgag | taatgtgtag | gtaaagattc | aaacgggtga | 650 |
| 651 | gaaaggccgg | agacagtcaa | atcaccatca | atatgatatt | caaccgttct | 700 |
| 701 | agctgataaa | ttcatgccgg | agagggtagc | tatttttgag | aggtctctac | 750 |
| 751 | aaaggctatc | aggtcattgc | ctgagagtct | ggagcaaaca | agagaatcga | 800 |
| 801 | tgaacggtaa | tcgtaaaact | agcatgtcaa | tcatatgtac | cccggttgat | 850 |
| 851 | aatcagaaaa | gccccaaaaa | caggaagatt | gtataagcaa | atatttaaat | 900 |
| 901 | tgtaaacgtt | aatattttgt | taaaattcgc | gttaaatttt | tgttaaatca | 950 |
| 951 | gctcattttt | taaccaatag | gccgaaatcg | gcaaaatccc | ttataaatca | 1000 |
| 1001 | aaagaataga | ccgagatagg | gttgagtgtt | gttccagttt | ggaacaagag | 1050 |
| 1051 | tccactatta | aagaacgtgg | actccaacgt | caaagggcga | aaaaccgtct | 1100 |
| 1101 | atcagggcga | tggcccacta | cgtgaaccat | caccctaatc | aagttttttg | 1150 |
| 1151 | gggtcgaggt | gccgtaaagc | actaaatcgg | aaccctaaag | ggagcccccg | 1200 |
| 1201 | atttagagct | tgacggggaa | agccggcgaa | cgtggcgaga | aaggaaggga | 1250 |
| 1251 | agaaagcgaa | aggagcgggc | gctagggcgc | tggcaagtgt | agcggtcacg | 1300 |
| 1301 | ctgcgcgtaa | ccaccacacc | cgccgcgctt | aatgcgccgc | tacagggcgc | 1350 |
| 1351 | gtactatggt | tgctttgacg | agcacgtata | acgtgctttc | ctcgttagaa | 1400 |
| 1401 | tcagagcggg | agctaaacag | gaggccgatt | aaagggattt | tagacaggaa | 1450 |
| 1451 | cggtacgcca | gaatcctgag | aagtgttttt | ataatcagtg | aggccaccga | 1500 |
| 1501 | gtaaaagagt | ctgtccatca | cgcaaattaa | ccgttgtcgc | aatacttctt | 1550 |
| 1551 | tgattagtaa | taacatcact | tgcctgagta | gaagaactca | aactatcggc | 1600 |
| 1601 | cttgctggta | atatccagaa | caatattacc | gccagccatt | gcaacaggaa | 1650 |
| 1651 | aaacgctcat | ggaaatacct | acattttgac | gctcaatcgt | ctggaanttc | 1700 |
| 1701 | ctgatgcggt | attttctcct | tacgcatctg | tgcggtattt | cacaccgcat | 1750 |
| 1751 | atggtgcact | ctcagtacaa | tctgctctga | tgccgcatag | ttaagccagc | 1800 |
| 1801 | cccgacaccc | gccaacaccc | gctgacgcgc | cctgacgggc | ttgtctgctc | 1850 |
| 1851 | ccggcatccg | cttacagaca | agctgtgacc | gtctccggga | gctgcatgtg | 1900 |
| 1901 | tcagaggttt | tcaccgtcat | caccgaaacg | cgcgagacga | aagggcctcg | 1950 |
| 1951 | tgatacgcct | atttttatag | gttaatgtca | tgataataat | ggtttcttag | 2000 |
| 2001 | acgtcaggtg | gcacttttcg | gggaaatgtg | cgcggaaccc | ctatttgttt | 2050 |
| 2051 | atttttctaa | atacattcaa | atatgtatcc | gctcatgaga | caataaccct | 2100 |
| 2101 | gataaatgct | tcaataatat | tgaaaaagga | agagtatgag | tattcaacat | 2150 |
| 2151 | ttccgtgtcg | cccttattcc | cttttttgcg | gcattttgcc | ttcctgtttt | 2200 |
| 2201 | tgctcaccca | gaaacgctgg | tgaaagtaaa | agatgctgaa | gatcagttgg | 2250 |
| 2251 | gtgcacgagt | gggttacatc | gaactggatc | tcaacagcgg | taagatcctt | 2300 |
| 2301 | gagagttttc | gccccgaaga | acgttttcca | atgatgagca | cttttaaagt | 2350 |
| 2351 | tctgctatgt | ggcgcggtat | tatcccgtat | tgacgccggg | caagagcaac | 2400 |
| 2401 | tcggtcgccg | catacactat | tctcagaatg | acttggttga | gtactcacca | 2450 |
| 2451 | gtcacagaaa | agcatcttac | ggatggcatg | acagtaagag | aattatgcag | 2500 |
| 2501 | tgctgccata | accatgagtg | ataacactgc | ggccaactta | cttctgacaa | 2550 |
| 2551 | cgatcggagg | accgaaggag | ctaaccgctt | ttttgcacaa | catgggggat | 2600 |
| 2601 | catgtaactc | gccttgatcg | ttgggaaccg | gagctgaatg | aagccatacc | 2650 |
| 2651 | aaacgacgag | cgtgacacca | cgatgcctgt | agcaatggca | acaacgttgc | 2700 |
| 2701 | gcaaactatt | aactggcgaa | ctacttactc | tagcttcccg | gcaacaatta | 2750 |
| 2751 | atagactgga | tggaggcgga | taaagttgca | ggaccacttc | tgcgctcggc | 2800 |
| 2801 | ccttccggct | ggctggttta | ttgctgataa | atctggagcc | ggtgagcgtg | 2850 |
| 2851 | ggtctcgcgg | tatcattgca | gcactggggc | cagatggtaa | gccctcccgt | 2900 |
| 2901 | atcgtagtta | tctacacgac | ggggagtcag | gcaactatgg | atgaacgaaa | 2950 |
| 2951 | tagacagatc | gctgagatag | gtgcctcact | gattaagcat | tggtaactgt | 3000 |
| 3001 | cagaccaagt | ttactcatat | atactttaga | ttgatttaaa | acttcatttt | 3050 |
| 3051 | taatttaaaa | ggatctaggt | gaagatcctt | tttgataatc | tcatgaccaa | 3100 |
| 3101 | aatcccttaa | cgtgagtttt | cgttccactg | agcgtcagac | cccgtagaaa | 3150 |
| 3151 | agatcaaagg | atcttcttga | gatccttttt | ttctgcgcgt | aatctgctgc | 3200 |
| 3201 | ttgcaaacaa | aaaaaccacc | gctaccagcg | gtggtttgtt | tgccggatca | 3250 |
| 3251 | agagctacca | actctttttc | cgaaggtaac | tggcttcagc | agagcgcaga | 3300 |
| 3301 | taccaaatac | tgtccttcta | gtgtagccgt | agttaggcca | ccacttcaag | 3350 |
| 3351 | aactctgtag | caccgcctac | atacctcgct | ctgctaatcc | tgttaccagt | 3400 |
| 3401 | ggctgctgcc | agtggcgata | agtcgtgtct | taccgggttg | gactcaagac | 3450 |
| 3451 | gatagttacc | ggataaggcg | cagcggtcgg | gctgaacggg | gggttcgtgc | 3500 |
| 3501 | acacagccca | gcttggagcg | aacgacctac | accgaactga | gatacctaca | 3550 |
| 3551 | gcgtgagcta | tgagaaagcg | ccacgcttcc | cgaagggaga | aaggcggaca | 3600 |
| 3601 | ggtatccggt | aagcggcagg | gtcggaacag | gagagcgcac | gagggagctt | 3650 |
| 3651 | ccagggggaa | acgcctggta | tctttatagt | cctgtcgggt | ttcgccacct | 3700 |
| 3701 | ctgacttgag | cgtcgatttt | tgtgatgctc | gtcagggggg | cggagcctat | 3750 |
| 3751 | ggaaaaacgc | cagcaacgcg | gcctttttac | ggttcctggc | cttttgctgg | 3800 |
| 3801 | ccttttgctc | acatgttctt | tcctgcgtta | tcccctgatt | ctgtggataa | 3850 |
| 3851 | ccgtattacc | gcctttgagt | gagctgatac | cgctcgccgc | agccgaacga | 3900 |
| 3901 | ccgagcgcag | cgagtcagtg | agcgaggaag | cggaagagc | 3939 |
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文献和实验Preparation of ssDNA from Phagemid Vectors
filamentous phage is packaged as ssDNA when the host cells are super-infected with helper phage. The first generation of phagemids, the pEMBL vectors, however, gave poor and n-reproducible ssDNA yields. This problem was resolved in two main ways
细胞中，也有好几种病毒复制被用来组建克隆载体。 3) 混合型载体：这类载体复制起点来自质粒和病毒，比如适合于大肠杆菌的DNA载体pEMBL8和pEMBL9含有质粒pMB1和单链噬菌体f1复制起点。 用于动物细胞的DNA载体则含有大肠杆菌的质粒复制和动物病毒复制起点。 使用得最广泛的动物病毒复制起点来源子SV40。实际上，这类混合型载体也是穿梭型的，即它们既可以在大肠杆菌中复制，亦可在动物细胞中复制。 要是按照克隆载体的功能或用途来划分，则可将DNA载体分为以下两大类： 1) 普通型载体：这类
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