Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Product Name:Caspase 9 Mouse Monoclonal Antibody
Clone Number: BE-21
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:1,000-5,000 IP: 1:200 IF: 1:100-200
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous Caspase 9 protein.
Alternative Names:APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody
Molecular Weight:47KD(H), 51KD(R, M)
Form: Liquid
Reactivity:H, R, M
Western blot analysis of Hela with Caspase 9 (BE-21) Mouse mAb dilutedat
1)1:2,000
2)1:5,000
Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (BE-21) Mouse mAb diluted at 1:3,000
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis:primary antibody:BE3111 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(BE0105), 1:5,000
IF analysis of Hela with BE3111(Left) and DAPI (Right) diluted at 1:100.