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- EASYBIO
- BE3145-50
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
GFAP Mouse Monoclonal Antibody
- 规格：
支
GFAP is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Product Name:GFAP Mouse Monoclonal Antibody
Clone Number: 6C9
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2000-5,000 IHC: 1:200
Optimal dilutions should be determined by the end user.
Specificity: The GFAP Mouse Monoclonal antibody detects endogenous GFAP proteins.
Molecular Weight:45KD
Form: Liquid
Reactivity:R,M
Western blot analysis of Rat Brain Tissue with GFAP mAb diluted at 1:5,000.
Western blot analysis of Mouse Brain Tissue with GFAP mAb diluted at 1:2,000.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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