- ¥400
- EASYBIO
- BE3393-10
- 2026年01月09日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Active Caspase 3 Mouse Monoclonal Antibody
- 规格：
10μl/支
Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position
Product Name:Active Caspase 3 Mouse Monoclonal Antibody
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:500-1,000
Optimal dilutions should be determined by the end user.
Specificity:Antibody can detects endogenous Active Caspase 3 protein.
Alternative Names:APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody
Form: Liquid
Reactivity: H, R, M
Molecular Weight: 17KD
Western blot analysis of 1) Hela, 2)3T3, 3)Rat Brain Tissue with Active Caspase 3 Mouse mAb BE3393 diluted at 1:1,000.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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