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- EASYBIO
- BE3003-50
- 2026年01月01日
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- 详细信息
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Histone H3(di methyl K27) Mouse Monoclonal Antibody
- 规格：
支
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Product Name:Histone H3 (di methyl K27)Mouse Monoclonal Antibody
Clone Number: 4B11
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1: 1,000-3,000 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous Histone H3 (di methyl K27)protein.
Alternative Names:H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Molecular Weight:15KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of Hela with Histone H3 (di methyl K27) (4B11) Mouse MAb diluted at
1) 1:1,000
2) 1:3,000
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : BE3003 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) (BE0102) 1:10,000
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
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