- ￥200
- EASYBIO
- BE2026-10
- 2025年12月28日
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- 详细信息
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- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
mCherry-Tag Mouse Monoclonal Antibody
- 规格：
10μl/支
Product Name Anti-mCherry monoclonal antibody
Clone Number: 7B4
Immunogen: Recombinant mCherry expressed in E.coli
Purification: Protein G affinity purification
Isotype: IgG1
Tested applications: WB IF.
Recommended dilutions: WB: 1:2000-5000 IF: 1:200
Optimal dilutions/concentrations should be determined by the end user.
Form:Liquid
Concentration:1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: mCherry antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Reactivity:ALL
PET-28a mCherry recombinant protein expressed in E.coli
Primary antibody Anti- mCherry mAB (EASYBIO BE2026)
Lane 1=1:5000 lane 2=1:10000
Secondary antibody Goat-anti mouse IgG (H&L) HRP (BE0102)1:10000
IF Test: Primary antibody dilution at 1:500
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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