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- EASYBIO
- BE0034-100
- 2025年12月25日
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- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
GAPDH(HRP Conjugated) Mouse Monoclonal Antibody
- 规格：
支
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Product Name:GAPDH Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 2B8
Storage Buffer :PBS, pH 7.4, containing 0.02% kathonas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2,000-5,000 IHC: 1:100
Optimal dilutions should be determined by the end user.
Specificity: The GAPDH antibody can detects endogenous GAPDH protein.
Alternative Names:G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphoate dehycdrogenase, MGC102544
Molecular Weight: 37KD
Form: Liquid
Reactivity:H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
Western blot analysis of Hela with BE0034 diluted at 1:3,000
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文献和实验Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，但是进口品牌如Abcam、Millipore、CST等价格偏高，一般在￥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体，分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody （货号E021010 ）是小鼠源的单抗，其效价很高，厂商推荐的WB起始稀释比率为1:1000，实际应用过程中，有使用1:10000或更高的报告，远远高于此稀释比率，说明其免疫效价很高，另外，其检测种属也很多，包括人、牛、猪、小鼠
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