Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Product Name:GAPDH Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 2B8
Storage Buffer :PBS, pH 7.4, containing 0.02% kathonas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2,000-5,000 IHC: 1:100
Optimal dilutions should be determined by the end user.
Specificity: The GAPDH antibody can detects endogenous GAPDH protein.
Alternative Names:G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphoate dehycdrogenase, MGC102544
Molecular Weight: 37KD
Form: Liquid
Reactivity:H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
Western blot analysis of Hela with BE0034 diluted at 1:3,000