10m Conical Centrifuge Tubes w

Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)

实验试剂   1. Absolute ethanol (96%-100%) 实验设备   1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes

A quick RNA mini-prep for Neurospora mycelial cultures

at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure

Methods for DNA isolation

for 5 minutes in 50 ml conical tubes in the Beckman GPR tabletop centrifuge and decant the supernatant. The cell pellets can be frozen at -70degC at this point. 3. Resuspend the cell pellets in 2 ml of GET/Lysozyme solution, add 4 ml of alkaline lysis