BAC克隆试剂盒BAC Cloning Kit

· 有效克隆任何大小在200kb的片段

· 从A/T或G/C丰富的基因组创建文库

· 高插入稳定性

· 诱导拷贝数

Lucigen's CopyRight® v2.0 BAC克隆试剂盒带有pSMART® BAC (Figure1)或pEZ BAC (Figure 2) 载体和适用于BAC文库优化的感受态细胞，确保文库的准确性，可靠性和有效性。这些载体整合了CloneSmart®无转录技术，最小化克隆的偏好性，有利于克隆产毒素基因。随着诱导拷贝数的扩增（20到50倍）可获得更高的DNA量。LacZ-sacB片段可以消除未切割载体所形成的假阳性克隆。不像其他BAC克隆系统，Lucigen's CloneSmart技术确保了未克隆化的插入也能稳定遗传。在克隆过程中，序列不容易发生丢失或重排。CopyRigh盒子与其他同类盒子相比，以较好的价格保证了最优的BAC克隆。图3显示了一个用pSMART BAC 载体构建的随机剪切的BAC文库。

Figure 1. CopyRight vector pSMART BAC.

Figure 2. CopyRight vector pEZ BAC.

Figure 3. 用pSMART BAC载体构建的拟南芥的随机剪切BAC文库.

易于成功
有效的获得克隆或创建文库。the CopyRight v2.0克隆盒子消除了繁琐的载体和感受态细胞制备过程，也省去了QC试验的耗时。盒子包括最优化的试剂，连接用载体，高效感受态细胞，详细说明书和疑惑解答。

每一个CopyRight® v2.0 BAC克隆盒子都包括：切割载体（pSMART BAC带有BamHI 或HindIII位点），CopyRight DNA连接酶，CopyRight 5X连接buffer，阳性对照插入DNA,测序引物，BAC优化的Replicator v2.0电转感受态细胞（一管可做两次转化），复苏培养基，阿拉伯糖诱导溶液和操作手册。

每一个CopyRight pEZ BAC克隆盒子含有: 切割CopyRight载体（pEZ BAC 平端）CloneSmart DNA连接酶，CloneDirect™10 X连接buffer（包括ATP）,阳性对照插入DNA，拷贝诱导溶液，测序引物和操作手册。盒子所带细胞还包括诱导BAC最适的Replicator v2.0感受态细胞（>1 × 10¹⁰ cfu/µg DNA），每管可做两次转化，阳性对照质粒和复苏培养基。

相关产品货号：

Product Description		Size	Cat. No.
BAC-Optimized Replicator v2.0 Electrocompetent Cells		12 rxns (DUOs)	60210-1
		24 rxns (DUOs)	60210-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC BamHI w/BAC-Optimized Replicator v2.0 Electrocompetent Cells		10 rxns	42030-1
		20 rxns	42030-2
CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells		10 rxns	42032-1
		20 rxns	42032-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells		10 rxns	42009-1
		20 rxns	42009-2
CopyRight v2.0 pEZ BAC Blunt Cloning Kit without cells		10 rxns	42016-1

Cloning Kits & Vectors

Lucigen has a wide variety of cloning systems, including novel transcription-free cloning vectors, that improve cloning yields, reduce cloning bias, and improve sequencing throughput. For cloning the most difficult sequences, the BigEasy® v2.0 Linear Cloning System offers unmatched stability. Most Lucigen cloning vectors are pre-cut, dephosphorylated, and ready-to-use.

Go directly to Vector Sequences >

Also see Protein Expression >

General Cloning

CloneSmart® Cloning Kits (pSMART® Vectors) » Stably clone any insert in transcription-free, blunt vectors for general cloning. Choice of antibiotic resistance and copy number.

GC Cloning and Amplification (pGC™ Blue Vector) » Clone PCR products with optional blue/white screening; includes T7 & SP6 RNA polymerase promoters.


Difficult Cloning or BAC/Fosmid Cloning

BigEasy® v2.0 Linear Cloning System (pJAZZ® Vectors) » Clone the unclonable, including large or highly repetitive DNA, solely available from Lucigen.

CopyControl™ Fosmid Library Production Kits » Efficiently clone any insert up to 40 kb with a complete kit, including packaging extracts.

MaxPlax™ Lambda Packaging Extracts » Package fosmid, cosmid or lambda DNA to generate genomic libraries.

CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC) » Efficiently clone any insert 200kb.

CopyRight® v2.0 Fosmid Cloning Kits (pSMART® FOS) » Efficiently clone any insert 35-45kb.


其他克隆相关试剂盒:

DNATerminator® End Repair Kit » Repair sheared or restriction-digested DNA to create blunt, 5' phosphorylated ends.

CloneDirect™ Rapid Ligation Kit » Proceed from ligation reaction to transformation in minutes.

Gel-Ready™ DNA Ladders » Bench-top stable markers — always ready to use.

End-It™ DNA End-Repair Kit » Prepare blunt, phosphorylated DNA ends from PCR product or sheared DNA.

GELase™ Agarose Gel-Digesting Preparation » Recover DNA and RNA from low-melting point agarose gels.

Fast-Link™ DNA Ligation Kit » Ligate DNA for cloning applications.