Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation. *Blocking peptide 可以联系我们购买。¥300/1ml
免疫原
This antibody has been raised in rabbit against two KLH-conjugated synthetic peptides containing an unmodified sequence from the central part and from the C-terminus of histone H3, respectively.
抗体规格
100μl
产品详情
克隆
Polyclonal
产品形态
Liquid
内容
Whole antiserum from rabbit containing 0.05% azide. *可以提供无载体抗体。
储存条件
At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
Core component of nucleosome. Nucleosomes wrap andcompact DNA into chromatin, limiting DNA accessibility to thecellular machineries which require DNA as a template. Histonesthereby play a central role in transcription regulation, DNArepair, DNA replication and chromosomal stability. DNAaccessibility is regulated via a complex set of post-translationalmodifications of histones, also called histone code, andnucleosome remodeling.
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a central role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes.
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[list_product_images]To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti- H3pan polyclonal antibody (Catalog # CI1025). By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:900,000.[/list_product_images]