Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
纯化方式
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
浓度
500 ug/ml
产品形态
Liquid
保存条件
At -20°C for one year. At 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Figure 2. Immunohistochemistry validation of CLDN7 using Anti-Claudin-7 (P202) CLDN7 Antibody (A03851-1).
Immunohistochemistry (IHC) analyzes of Claudin-7 (P202) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC|
Figure 1. Western blotting validation for Anti-Claudin-7 (P202) CLDN7 Antibody A03851-1
Western blot (WB) analysis of Claudin-7 (T202) polyclonal antibody at 1:500 dilution
Lane1:HCC827 whole cell lysate(40ug)
Lane2:The Lung tissue lysate of Mouse(40ug)
Lane3:The Lung tissue lysate of Rat(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:L02 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration|
Figure 3. Western blotting validation for Anti-Claudin-7 (P202) CLDN7 Antibody A03851-1
Immunoprecipitation of Hela cell lysate using Claudin-7 (P202) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 and lane 5 ) .Lane 1 is 30% input. The western blot was probed using Claudin-7 (P202) #A03851-1. “↑”锛坰upernatant锛? “↓(deposition锛?br>Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration[/list_product_images]