- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
p15TV-L(p15TVL)
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: p15TV-L
其他名称:
质粒大小(bp): 7737
抗性: Amp
质粒类型: Bacterial Expression
是否病毒: unspecified
启动子: unspecified
稳转或瞬转: unspecified
组成型或诱导性: unspecified
表达水平: High Copy
蛋白标签: Hexahistidine
测序引物:
GenBank号:
其他属性:
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| AmpR_promoter | 139 | 167 | |
| Ampicillin | 209 | 1069 | |
| pBR322_origin | 1224 | 1843 | |
| pGEX_3_primer | 2242 | 2264 | |
| ROP | 2449 | 2258 | |
| tet (611 – 0) | 3699 | 3088 | |
| lacI | 4852 | 3761 | |
| tet (887 – 636) | 5195 | 4944 | |
| pBRrevBam_primer | 5127 | 5146 | |
| T7_promoter | 5230 | 5248 | |
| lacO | 5248 | 5275 | |
| T7_transl_en_RBS | 5293 | 5309 | |
| loxP | 5398 | 5431 | |
| sacB_neg | 5440 | 7350 | |
| T7_terminator | 7422 | 7550 | |
| T7_Terminal_primer | 7482 | 7464 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 209 | 1069 | |
| ORF frame 1 | 3705 | 3088 | |
| ORF frame 1 | 3283 | 3978 | |
| ORF frame 3 | 4720 | 3761 | |
| ORF frame 2 | 5093 | 4593 | |
| ORF frame 3 | 5892 | 7313 |
| Enzyme Name | Cut |
|---|---|
| PstI | 754 |
| NruI | 3389 |
| ApaI | 4285 |
| BclI | 4474 |
| BglII | 5210 |
| XbaI | 5276 |
| NdeI | 5372 |
| StuI | 6133 |
| KpnI | 6953 |
| SacII | 6971 |
| XhoI | 7409 |
| BamHI | 7414 |
| EcoRI | 7735 |
质粒序列:
| 1 | ttcttgaaga | cgaaagggcc | tcgtgatacg | cctattttta | taggttaatg | 50 |
| 51 | tcatgataat | aatggtttct | tagacgtcag | gtggcacttt | tcggggaaat | 100 |
| 101 | gtgcgcggaa | cccctatttg | tttatttttc | taaatacatt | caaatatgta | 150 |
| 151 | tccgctcatg | agacaataac | cctgataaat | gcttcaataa | tattgaaaaa | 200 |
| 201 | ggaagagtat | gagtattcaa | catttccgtg | tcgcccttat | tccctttttt | 250 |
| 251 | gcggcatttt | gccttcctgt | ttttgctcac | ccagaaacgc | tggtgaaagt | 300 |
| 301 | aaaagatgct | gaagatcagt | tgggtgcacg | agtgggttac | atcgaactgg | 350 |
| 351 | atctcaacag | cggtaagatc | cttgagagtt | ttcgccccga | agaacgtttt | 400 |
| 401 | ccaatgatga | gcacttttaa | agttctgcta | tgtggcgcgg | tattatcccg | 450 |
| 451 | tgttgacgcc | gggcaagagc | aactcggtcg | ccgcatacac | tattctcaga | 500 |
| 501 | atgacttggt | tgagtactca | ccagtcacag | aaaagcatct | tacggatggc | 550 |
| 551 | atgacagtaa | gagaattatg | cagtgctgcc | ataaccatga | gtgataacac | 600 |
| 601 | tgcggccaac | ttacttctga | caacgatcgg | aggaccgaag | gagctaaccg | 650 |
| 651 | cttttttgca | caacatgggg | gatcatgtaa | ctcgccttga | tcgttgggaa | 700 |
| 701 | ccggagctga | atgaagccat | accaaacgac | gagcgtgaca | ccacgatgcc | 750 |
| 751 | tgcagcaatg | gcaacaacgt | tgcgcaaact | attaactggc | gaactactta | 800 |
| 801 | ctctagcttc | ccggcaacaa | ttaatagact | ggatggaggc | ggataaagtt | 850 |
| 851 | gcaggaccac | ttctgcgctc | ggcccttccg | gctggctggt | ttattgctga | 900 |
| 901 | taaatctgga | gccggtgagc | gtgggtctcg | cggtatcatt | gcagcactgg | 950 |
| 951 | ggccagatgg | taagccctcc | cgtatcgtag | ttatctacac | gacggggagt | 1000 |
| 1001 | caggcaacta | tggatgaacg | aaatagacag | atcgctgaga | taggtgcctc | 1050 |
| 1051 | actgattaag | cattggtaac | tgtcagacca | agtttactca | tatatacttt | 1100 |
| 1101 | agattgattt | aaaacttcat | ttttaattta | aaaggatcta | ggtgaagatc | 1150 |
| 1151 | ctttttgata | atctcatgac | caaaatccct | taacgtgagt | tttcgttcca | 1200 |
| 1201 | ctgagcgtca | gaccccgtag | aaaagatcaa | aggatcttct | tgagatcctt | 1250 |
| 1251 | tttttctgcg | cgtaatctgc | tgcttgcaaa | caaaaaaacc | accgctacca | 1300 |
| 1301 | gcggtggttt | gtttgccgga | tcaagagcta | ccaactcttt | ttccgaaggt | 1350 |
| 1351 | aactggcttc | agcagagcgc | agataccaaa | tactgtcctt | ctagtgtagc | 1400 |
| 1401 | cgtagttagg | ccaccacttc | aagaactctg | tagcaccgcc | tacatacctc | 1450 |
| 1451 | gctctgctaa | tcctgttacc | agtggctgct | gccagtggcg | ataagtcgtg | 1500 |
| 1501 | tcttaccggg | ttggactcaa | gacgatagtt | accggataag | gcgcagcggt | 1550 |
| 1551 | cgggctgaac | ggggggttcg | tgcacacagc | ccagcttgga | gcgaacgacc | 1600 |
| 1601 | tacaccgaac | tgagatacct | acagcgtgag | ctatgagaaa | gcgccacgct | 1650 |
| 1651 | tcccgaaggg | agaaaggcgg | acaggtatcc | ggtaagcggc | agggtcggaa | 1700 |
| 1701 | caggagagcg | cacgagggag | cttccagggg | gaaacgcctg | gtatctttat | 1750 |
| 1751 | agtcctgtcg | ggtttcgcca | cctctgactt | gagcgtcgat | ttttgtgatg | 1800 |
| 1801 | ctcgtcaggg | gggcggagcc | tatggaaaaa | cgccagcaac | gcggcctttt | 1850 |
| 1851 | tacggttcct | ggccttttgc | tggccttttg | ctcacatgtt | ctttcctgcg | 1900 |
| 1901 | ttatcccctg | attctgtgga | taaccgtatt | accgcctttg | agtgagctga | 1950 |
| 1951 | taccgctcgc | cgcagccgaa | cgaccgagcg | cagcgagtca | gtgagcgagg | 2000 |
| 2001 | aagcggaaga | gcgcctgatg | cggtattttc | tccttacgca | tctgtgcggt | 2050 |
| 2051 | atttcacacc | gcatatatgg | tgcactctca | gtacaatctg | ctctgatgcc | 2100 |
| 2101 | gcatagttaa | gccagtatac | actccgctat | cgctacgtga | ctgggtcatg | 2150 |
| 2151 | gctgcgcccc | gacacccgcc | aacacccgct | gacgcgccct | gacgggcttg | 2200 |
| 2201 | tctgctcccg | gcatccgctt | acagacaagc | tgtgaccgtc | tccgggagct | 2250 |
| 2251 | gcatgtgtca | gaggttttca | ccgtcatcac | cgaaacgcgc | gaggcagctg | 2300 |
| 2301 | cggtaaagct | catcagcgtg | gtcgtgaagc | gattcacaga | tgtctgcctg | 2350 |
| 2351 | ttcatccgcg | tccagctcgt | tgagtttctc | cagaagcgtt | aatgtctggc | 2400 |
| 2401 | ttctgataaa | gcgggccatg | ttaagggcgg | ttttttcctg | tttggtcact | 2450 |
| 2451 | gatgcctccg | tgtaaggggg | atttctgttc | atgggggtaa | tgataccgat | 2500 |
| 2501 | gaaacgagag | aggatgctca | cgatacgggt | tactgatgat | gaacatgccc | 2550 |
| 2551 | ggttactgga | acgttgtgag | ggtaaacaac | tggcggtatg | gatgcggcgg | 2600 |
| 2601 | gaccagagaa | aaatcactca | gggtcaatgc | cagcgcttcg | ttaatacaga | 2650 |
| 2651 | tgtaggtgtt | ccacagggta | gccagcagca | tcctgcgatg | cagatccgga | 2700 |
| 2701 | acataatggt | gcagggcgct | gacttccgcg | tttccagact | ttacgaaaca | 2750 |
| 2751 | cggaaaccga | agaccattca | tgttgttgct | caggtcgcag | acgttttgca | 2800 |
| 2801 | gcagcagtcg | cttcacgttc | gctcgcgtat | cggtgattca | ttctgctaac | 2850 |
| 2851 | cagtaaggca | accccgccag | cctagccggg | tcctcaacga | caggagcacg | 2900 |
| 2901 | atcatgcgca | cccgtggcca | ggacccaacg | ctgcccgaga | tgcgccgcgt | 2950 |
| 2951 | gcggctgctg | gagatggcgg | acgcgatgga | tatgttctgc | caagggttgg | 3000 |
| 3001 | tttgcgcatt | cacagttctc | cgcaagaatt | gattggctcc | aattcttgga | 3050 |
| 3051 | gtggtgaatc | cgttagcgag | gtgccgccgg | cttccattca | ggtcgaggtg | 3100 |
| 3101 | gcccggctcc | atgcaccgcg | acgcaacgcg | gggaggcaga | caaggtatag | 3150 |
| 3151 | ggcggcgcct | acaatccatg | ccaacccgtt | ccatgtgctc | gccgaggcgg | 3200 |
| 3201 | cataaatcgc | cgtgacgatc | agcggtccag | tgatcgaagt | taggctggta | 3250 |
| 3251 | agagccgcga | gcgatccttg | aagctgtccc | tgatggtcgt | catctacctg | 3300 |
| 3301 | cctggacagc | atggcctgca | acgcgggcat | cccgatgccg | ccggaagcga | 3350 |
| 3351 | gaagaatcat | aatggggaag | gccatccagc | ctcgcgtcgc | gaacgccagc | 3400 |
| 3401 | aagacgtagc | ccagcgcgtc | ggccgccatg | ccggcgataa | tggcctgctt | 3450 |
| 3451 | ctcgccgaaa | cgtttggtgg | cgggaccagt | gacgaaggct | tgagcgaggg | 3500 |
| 3501 | cgtgcaagat | tccgaatacc | gcaagcgaca | ggccgatcat | cgtcgcgctc | 3550 |
| 3551 | cagcgaaagc | ggtcctcgcc | gaaaatgacc | cagagcgctg | ccggcacctg | 3600 |
| 3601 | tcctacgagt | tgcatgataa | agaagacagt | cataagtgcg | gcgacgatag | 3650 |
| 3651 | tcatgccccg | cgcccaccgg | aaggagctga | ctgggttgaa | ggctctcaag | 3700 |
| 3701 | ggcatcggtc | gagatcccgg | tgcctaatga | gtgagctaac | ttacattaat | 3750 |
| 3751 | tgcgttgcgc | tcactgcccg | ctttccagtc | gggaaacctg | tcgtgccagc | 3800 |
| 3801 | tgcattaatg | aatcggccaa | cgcgcgggga | gaggcggttt | gcgtattggg | 3850 |
| 3851 | cgccagggtg | gtttttcttt | tcaccagtga | gacgggcaac | agctgattgc | 3900 |
| 3901 | ccttcaccgc | ctggccctga | gagagttgca | gcaagcggtc | cacgctggtt | 3950 |
| 3951 | tgccccagca | ggcgaaaatc | ctgtttgatg | gtggttaacg | gcgggatata | 4000 |
| 4001 | acatgagctg | tcttcggtat | cgtcgtatcc | cactaccgag | atatccgcac | 4050 |
| 4051 | caacgcgcag | cccggactcg | gtaatggcgc | gcattgcgcc | cagcgccatc | 4100 |
| 4101 | tgatcgttgg | caaccagcat | cgcagtggga | acgatgccct | cattcagcat | 4150 |
| 4151 | ttgcatggtt | tgttgaaaac | cggacatggc | actccagtcg | ccttcccgtt | 4200 |
| 4201 | ccgctatcgg | ctgaatttga | ttgcgagtga | gatatttatg | ccagccagcc | 4250 |
| 4251 | agacgcagac | gcgccgagac | agaacttaat | gggcccgcta | acagcgcgat | 4300 |
| 4301 | ttgctggtga | cccaatgcga | ccagatgctc | cacgcccagt | cgcgtaccgt | 4350 |
| 4351 | cttcatggga | gaaaataata | ctgttgatgg | gtgtctggtc | agagacatca | 4400 |
| 4401 | agaaataacg | ccggaacatt | agtgcaggca | gcttccacag | caatggcatc | 4450 |
| 4451 | ctggtcatcc | agcggatagt | taatgatcag | cccactgacg | cgttgcgcga | 4500 |
| 4501 | gaagattgtg | caccgccgct | ttacaggctt | cgacgccgct | tcgttctacc | 4550 |
| 4551 | atcgacacca | ccacgctggc | acccagttga | tcggcgcgag | atttaatcgc | 4600 |
| 4601 | cgcgacaatt | tgcgacggcg | cgtgcagggc | cagactggag | gtggcaacgc | 4650 |
| 4651 | caatcagcaa | cgactgtttg | cccgccagtt | gttgtgccac | gcggttggga | 4700 |
| 4701 | atgtaattca | gctccgccat | cgccgcttcc | actttttccc | gcgttttcgc | 4750 |
| 4751 | agaaacgtgg | ctggcctggt | tcaccacgcg | ggaaacggtc | tgataagaga | 4800 |
| 4801 | caccggcata | ctctgcgaca | tcgtataacg | ttactggttt | cacattcacc | 4850 |
| 4851 | accctgaatt | gactctcttc | cgggcgctat | catgccatac | cgcgaaaggt | 4900 |
| 4901 | tttgcgccat | tcgatggtgt | ccgggatctc | gacgctctcc | cttatgcgac | 4950 |
| 4951 | tcctgcatta | ggaagcagcc | cagtagtagg | ttgaggccgt | tgagcaccgc | 5000 |
| 5001 | cgccgcaagg | aatggtgcat | gcaaggagat | ggcgcccaac | agtcccccgg | 5050 |
| 5051 | ccacggggcc | tgccaccata | cccacgccga | aacaagcgct | catgagcccg | 5100 |
| 5101 | aagtggcgag | cccgatcttc | cccatcggtg | atgtcggcga | tataggcgcc | 5150 |
| 5151 | agcaaccgca | cctgtggcgc | cggtgatgcc | ggccacgatg | cgtccggcgt | 5200 |
| 5201 | agaggatcga | gatctcgatc | ccgcgaaatt | aatacgactc | actatagggg | 5250 |
| 5251 | aattgtgagc | ggataacaat | tcccctctag | aaataatttt | gtttaacttt | 5300 |
| 5301 | aagaaggaga | tataccatgc | atcatcatca | tcatcacagc | agcggcagag | 5350 |
| 5351 | aaaacttgta | tttccagggc | catatgagtt | ctcctcctga | aagatccata | 5400 |
| 5401 | acttcgtata | gcatacatta | tacgaagtta | tgcggccgcg | acgtccacat | 5450 |
| 5451 | atacctgccg | ttcactatta | tttagtgaaa | tgagatatta | tgatattttc | 5500 |
| 5501 | tgaattgtga | ttaaaaaggc | aactttatgc | ccatgcaaca | gaaactataa | 5550 |
| 5551 | aaaatacaga | gaatgaaaag | aaacagatag | attttttagt | tctttaggcc | 5600 |
| 5601 | cgtagtctgc | aaatcctttt | atgattttct | atcaaacaaa | agaggaaaat | 5650 |
| 5651 | agaccagttg | caatccaaac | gagagtctaa | tagaatgagg | tcgaaaagta | 5700 |
| 5701 | aatcgcgcgg | gtttgttact | gataaagcag | gcaagaccta | aaatgtgtaa | 5750 |
| 5751 | agggcaaagt | gtatactttg | gcgtcacccc | ttacatattt | taggtctttt | 5800 |
| 5801 | tttattgtgc | gtaactaact | tgccatcttc | aaacaggagg | gctggaagaa | 5850 |
| 5851 | gcagaccgct | aacacagtac | ataaaaaagg | agacatgaac | gatgaacatc | 5900 |
| 5901 | aaaaagtttg | caaaacaagc | aacagtatta | acctttacta | ccgcactgct | 5950 |
| 5951 | ggcaggaggc | gcaactcaag | cgtttgcgaa | agaaacgaac | caaaagccat | 6000 |
| 6001 | ataaggaaac | atacggcatt | tcccatatta | cacgccatga | tatgctgcaa | 6050 |
| 6051 | atccctgaac | agcaaaaaaa | tgaaaaatat | caagttcctg | agttcgattc | 6100 |
| 6101 | gtccacaatt | aaaaatatct | cttctgcaaa | aggcctggac | gtttgggaca | 6150 |
| 6151 | gctggccatt | acaaaacgct | gacggcactg | tcgcaaacta | tcacggctac | 6200 |
| 6201 | cacatcgtct | ttgcattagc | cggagatcct | aaaaatgcgg | atgacacatc | 6250 |
| 6251 | gatttacatg | ttctatcaaa | aagtcggcga | aacttctatt | gacagctgga | 6300 |
| 6301 | aaaacgctgg | ccgcgtcttt | aaagacagcg | acaaattcga | tgcaaatgat | 6350 |
| 6351 | tctatcctaa | aagaccaaac | acaagaatgg | tcaggttcag | ccacatttac | 6400 |
| 6401 | atctgacgga | aaaatccgtt | tattctacac | tgatttctcc | ggtaaacatt | 6450 |
| 6451 | acggcaaaca | aacactgaca | actgcacaag | ttaacgtatc | agcatcagac | 6500 |
| 6501 | agctctttga | acatcaacgg | tgtagaggat | tataaatcaa | tctttgacgg | 6550 |
| 6551 | tgacggaaaa | acgtatcaaa | atgtacagca | gttcatcgat | gaaggcaact | 6600 |
| 6601 | acagctcagg | cgacaaccat | acgctgagag | atcctcacta | cgtagaagat | 6650 |
| 6651 | aaaggccaca | aatacttagt | atttgaagca | aacactggaa | ctgaagatgg | 6700 |
| 6701 | ctaccaaggc | gaagaatctt | tatttaacaa | agcatactat | ggcaaaagca | 6750 |
| 6751 | catcattctt | ccgtcaagaa | agtcaaaaac | ttctgcaaag | cgataaaaaa | 6800 |
| 6801 | cgcacggctg | agttagcaaa | cggcgctctc | ggtatgattg | agctaaacga | 6850 |
| 6851 | tgattacaca | ctgaaaaaag | tgatgaaacc | gctgattgca | tctaacacag | 6900 |
| 6901 | taacagatga | aattgaacgc | gcgaacgtct | ttaaaatgaa | cggcaaatgg | 6950 |
| 6951 | tacctgttca | ctgactcccg | cggatcaaaa | atgacgattg | acggcattac | 7000 |
| 7001 | gtctaacgat | atttacatgc | ttggttatgt | ttctaattct | ttaactggcc | 7050 |
| 7051 | catacaagcc | gctgaacaaa | actggccttg | tgttaaaaat | ggatcttgat | 7100 |
| 7101 | cctaacgatg | taacctttac | ttactcacac | ttcgctgtac | ctcaagcgaa | 7150 |
| 7151 | aggaaacaat | gtcgtgatta | caagctatat | gacaaacaga | ggattctacg | 7200 |
| 7201 | cagacaaaca | atcaacgttt | gcgcctagct | tcctgctgaa | catcaaaggc | 7250 |
| 7251 | aagaaaacat | ctgttgtcaa | agacagcatc | cttgaacaag | gacaattaac | 7300 |
| 7301 | agttaacaaa | taaaaacgca | aaagaaaatg | ccgatatcct | attggcattg | 7350 |
| 7351 | acgtcaggtg | gcacttttcg | aggagatcat | gcacatgatg | acgaagcttg | 7400 |
| 7401 | cggccgcact | cgaggatccg | gctgctaaca | aagcccgaaa | ggaagctgag | 7450 |
| 7451 | ttggctgctg | ccaccgctga | gcaataacta | gcataacccc | ttggggcctc | 7500 |
| 7501 | taaacgggtc | ttgaggggtt | ttttgctgaa | aggaggaact | atatccggat | 7550 |
| 7551 | atcccgcaag | aggcccggca | gtaccggcat | aaccaagcct | atgcctacag | 7600 |
| 7601 | catccagggt | gacggtgccg | aggatgacga | tgagcgcatt | gttagatttc | 7650 |
| 7651 | atacacggtg | cctgactgcg | ttagcaattt | aactgtgata | aactaccgca | 7700 |
| 7701 | ttaaagctta | tcgatgataa | gctgtcaaac | atgagaa | 7737 |
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文献和实验一、SP 法 1)脱蜡、水化;2)PBS 洗 2~3 次各 5 分钟;3)3% H2O2(80% 甲醇)滴加在 TMA 上,室温静置 10 分钟;4)PBS 洗 2~3 次各 5 分钟;5)抗原修复;6)PBS 洗 2~3 次各5分钟;7)滴加正常山羊血清封闭液,室温 20 分钟。甩去多余液体。8)滴加Ⅰ抗 50μl,室温静置 1 小时或者4℃过夜或者37℃1小时。9)4℃ 过夜后需在37℃复温45分钟。10)PBS 洗 3 次各 5 分钟;11)滴加 Ⅱ 抗 40~50 μl,室温
HLA I 类基因区 HLA I类基因区位于复合体的最远端,即从端粒端的HCP5p15到MICB之间,长度为1.9Mb,包括122个基因座位,其中41个为表达基因,12个为后选基因,3个为非编码基因及66个假基因。根据编码产物分布、功能及多态性不同又可分为经典工类基因和非经典I类基因。 (1)经典HLA I类基因:又称HLA-Ia,是指最早发现的三个功能基因即HLA-A、HLA-月和HLA―C。HLA-Ia基因均具有高度多态性,如表4―l所示,截至2006年7月
-induced silencing complex (RISC), to mRNA. This process has been seen in a wide range of organisms such as Neurospora fungus (quelling), plants (post-transcriptional gene silencing), and mammalian cells (RNAi). Recent reports1 and 2 KV Morris, SW-L Chan
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