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1X10^6 cells
ATCC
详见说明书
详见说明书
细胞系
乳腺 乳房 导管
正常
人
不详
上皮细胞样
不详
乳腺 乳房 导管
冻成管或活细胞
贴壁生长
株
出品公司: | ATCC |
---|---|
细胞名称: | ATCC HTB-133(T-47D), T-47D, 人乳腺管癌细胞 |
存储人: | I Keydar |
种属来源: | 人 |
组织来源: | 乳腺 乳房 导管 |
疾病特征: | 来自导管癌肋膜渗出液转移灶 |
细胞形态: | 上皮细胞样 |
生长特性: | 贴壁生长 |
培养基: | DMEM培养基(GIBCO,货号12800017),90%;FBS,10%。 |
产品目录号: | HTB-133 |
生长条件: | 气相:空气,95%;二氧化碳,5%; 温度:37 ℃, |
传代方法: | 1:2至1:6,每周2次。 |
冻存条件: | 90% 完全培养基+10% DMSO,液氮储存 |
支原体检测: | 阴性 |
安全等级: | 1 |
应用: | 该细胞可以作为转染宿主细胞。 |
STR: |
Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
|
同工酶: |
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
|
参考文献: |
Judge SM, Chatterton RT Jr.. Progesterone-specific stimulation of triglyceride biosynthesis in a breast cancer cell line (T-47D). Cancer Res. 43: 4407-4412, 1983. PubMed: 6871874
Lamp SJ, et al. Calcitonin induction of a persistent activated state of adenylate cyclase in human breast cancer cells (T-47D). J. Biol. Chem. 256: 12269-12274, 1981. PubMed: 6271778
Sher E, et al. Whole-cell uptake and nuclear localization of 1,25-dihydroxy-cholecalciferol by breast cancer cells (T-47D) in culture. Biochem. J. 200: 315-320, 1981. PubMed: 6896147
Freake HC, et al. 1,25-Dihydroxyvitamin D3 specifically binds to a human breast cancer cell line (T-47D) and stimulates growth. Biochem. Biophys. Res. Commun. 101: 1131-1138, 1981. PubMed: 6272774
Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
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