产品名称:Anti-6X His tag® antibody [HIS.H8] 产品货号:Ab18184 产品规格:100ug 保存条件:保存于-20℃ 形态:液体 应用范围:ICC/IF, ELISA, Dot blot, WB, IP 保质期:按说明书条件可保存1-2年
Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution. Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa) Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer. Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein. The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa). Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184) ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
Aoki H et al. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA)n. Cerebellum 22:70-84 (2023).
Saville JW et al. Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein. Cell Rep 42:111964 (2023).
Hariharasudhan G et al. TOPORS-mediated RAD51 SUMOylation facilitates homologous recombination repair. Nucleic Acids Res 50:1501-1516 (2022).