cofilin(Phospho-Ser3) Antibody

别名:CFL; CFL1;
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

图片:

Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody #11139(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody #11139(left) or the same antibody preincubated with blocking peptide(right).

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Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody #11139.

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Lane 1 : Hela?treated with H2O2; Lane 2 : Mouse brain tissue; Lane 3 : Rat brain tissue; Lane 4 : N-peptide blocked Hela?treated with H2O2; Lane 5 : N-peptide blocked Mouse brain tissue; Lane 6 : N-peptide blocked Rat brain tissue; Lane 7 : P-peptide blocked Hela?treated with H2O2; Lane 8 : P-peptide blocked Mouse brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 μg per lane. Predicted band size :?19 kDa Observed band size :?19 kDa