- 询价
- Signalway Antibody
- 11056
- 2025年09月03日
- WB IHC IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC IF
- 靶点：
Uniprot：P60484
- 抗体英文名：
PTEN(Phospho-Ser380/Thr382/Thr383) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 54kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
图片:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056(left) or the same antibody preincubated with blocking peptide(right).
,
Immunofluorescence staining of methanol-fixed Hela cells using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056.
,
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody #11056.
,
Western blot analysis of extracts from Hela and C6 cells,treated with EGF or PMA, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056.
,
Immunofluorescence staining of methanol-fixed MEFcells using PTEN (Phospho-Ser380/Thr382/Thr383)Antibody #11056.
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文献和实验Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt
-dependent activation and phosphorylation of the survival kinase Akt can be used as readout for cellular PTEN activity. Here we have outlined a detailed procedure employing a phosphoserine-specific anti-Akt antibody to examine the content of phosphorylated
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
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