- 询价
- Signalway Antibody
- 38097
- 2025年09月22日
- WB IF
- Mouse
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB IF
- 靶点：
Uniprot：P08670
- 抗体英文名：
Vimentin Mouse Monoclonal Antibody(1A7)
- 规格：
50ul/100ul
配方:Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:1000~1:3000
Immunofluorescence: 1:100-200
图片:
Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue, using #38097 diluted at 1:2,000.
,
IF analysis of Hela with #38097(Left) and DAPI (Right) diluted at 1:100.
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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