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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human GSK3 alpha/beta
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB IHC
- 靶点:
Uniprot:P49840
- 抗体英文名:
GSK3 alpha/beta Antibody
- 规格:
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
WB: 1:500-1:2000
图片:

Western blot analysis of GSK3 alpha/beta expression in TNF-a treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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文献和实验真爱满行囊 我最近在体外细胞做的关于gsk3-beta功能问题,发现药物处理以后,gsk-3-beta的总表达量下调,如果是这样的话 1、我是否能够得出gsk-3-beta的活性下调的结论? 2、是否还有必要做非活性形式的表达量?我的理解是,基础状态下,gsk-3beta维持的是低活性状态,如果总量都下调了,那应该能够得出活性降低的结论了吧? 3、另外,在这种情况下我是否有必要去做gsk-3-beta的216位点的活性形式的表达
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Chem. 275, 32475– 32481. 11. Liu, X., Rubin, J. S., and Kimmel, A. R. (2005) Rapid, Wnt-induced changes in GSK3beta associations that regulate betacateninstabilization are mediated by Galpha proteins. Curr. Biol. 15, 1989–1997. 12. Cook, D., Fry
for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
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