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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 相关疾病：
鳞状细胞癌
- 物种来源：
人
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
| Designations: | RPMI 2650 | ||
| Depositors: | GE Moore | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: nasal septum Disease: squamous cell carcinoma Derived from metastatic site: pleural effusion |
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| Cellular Products: | mucoid; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The cells are positive for keratin by immunoperoxidase staining. | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,11 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,11 THO1: 6,8 TPOX: 8 vWA: 16,18 |
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| Isoenzymes: | G6PD, B | ||
| Age: | 52 years | ||
| Gender: | male | ||
| Comments: | The cells are positive for keratin by immunoperoxidase staining. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. | ||
| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37�C.NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22218: Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677 25971: Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238 |
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