SOB培养基（溶液）(pH7.0),SOB liquid m

超级感受态制备

by autoclaving for 20min at 15 lb/sq. in on liquid cycle Just before use, combine the medium with 1 ml of 2 M Mg2+ (20 mM final) E. SOC medium: SOC medium is identical to SOB medium, except that it contains 20 mM Glucose. 3) A autoclaved 500 ml flask

超级感受态细胞的制备

(1.05 kg/cm2 ) on liquid cycle.) SOB agar plates containing 20 mM MgSO4 and the appropriate antibiotic Standard SOB contains 10 mM MgSO4 . SOB medium, for growth of culture to be transformed Prepare

【共享】超高效电转化细胞的制备、电击及要点——经过实践检验可靠适用

PH7.0，灭菌后加入过滤除菌的MgCl2备用； 实际配制100mL时：2g bacto－trptone、0.5g bacto－yeast extract、0.05g NaCl完全溶加0.0186g KCl，10N NaOH一小滴即可调至PH7.0 分装入50mL培养基/500mL瓶高压灭菌；此后50mL中加入250uL 2M MgCl2即为SOB培养基； SOC：50mL无菌SOB培养基+1mL 1M 过滤除菌的葡萄糖即为SOC，分装1mL每管，冻存于-20备用。 10％甘油：用分子