| 感受态细胞名称: | BL21-SI, BL21SI |
|---|---|
| 菌株类型: | E.coli |
| 产品目录号: | 11665 |
| 培养基: | LB培养基 |
| 生长条件: | 37 ℃, 有氧 |
| 基因型: | F- ompT hsdSB(rB-, mB-) gal dcm endA1 lon- proUp::T7 RNAP::malQ-lacZ (TetS) |
| 抗性: | 四环素敏感 |
| 质粒转化条件: | 42 ℃ 热激 |
| 应用: | 蛋白表达 |
| 诱导方法: | IPTG |
| 菌株特点: | BL21SI是 lon和ompT蛋白酶缺陷型型菌株。 BL21-SI感受态细胞是用于蛋白质表达的宿主菌株。 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃
- 保质期:
6个月
- 英文名:
BL21-SI
- 库存:
大量现货
- 供应商:
信裕生物
- 规格:
10×100μl/50×100μl
BL21-SI感受态细胞
BL21SI感受态细胞基本信息
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文献和实验SI Mapping Using Single-Stranded DNA Probes
The SI nuclease is an endonuclease isolated from Aspergillus oryzae that digests single‐ but not doublesdeshstranded nucleic acid. In addition, it digests partially mismatched double‐stranded molecules with such sensitivity that even a single
approaches used for capillary coating and concentrates on those utilizing Si-O and Si-C covalent bonds. The apparatus and methods are presented for capillary coating using Si-O and Si-C chemistry. Furthermore, procedures are described for monitoring
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (dnEBNA-1/Soft)in E.coli BL21 LysS per 0.5L LB+ ampicillin (grow two 0.5L cultures of each) Incubate ~2hrs 37℃250rpm until OD600 = 0.4-0.6 Induce with 5ml 100mM ITPG
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