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- TABSRT01KIT
- 2025年07月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20°
- 保质期：
12个月
- 英文名：
TwistAmp Basic RT
- 库存：
1
- 供应商：
畅宇云商（北京）科技有限公司
- CAS号：
TABSRT01KIT
| TABSRT01KIT | TwistAmp™ Basic RT | 用于逆转录RPA反应 |
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp Basic用于逆转录反应，CDNA快速扩增. 除基本组分外, 还加有通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40-42°C)
3) Amplicons of 80-400bp are
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.4 μl
Primer B (10μM) 2.4 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
Vortex and spin briefly
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Incubate at 40-42°C for 20-40 minutes.
4b) For low template copy number,
remove strip after 5-7 minutes, invert
vigorously 8-10 times to mix & spin
briefly, replace in heating device.
5) After 20-40 minutes, clean amplicons before running on agarose gels.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
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文献和实验Basic RT-PCR for Measurement of Cytokine Expression
As an alternative to the ELISPOT assay described in previous chapters, the expression of known cytokines can be analyzed at the mRNA level. Although expression of mRNA for a particular cytokine does not imply corre-sponding expression
highlighted in red, and 5’ and 3’ UTR sequence in blue. Save the sequence in word or plain text (does not matter) but make sure you highlight each of these regions so you can go back and find them. It is important when you design primers for RT-PCR to detect
that you are detecting gets sandwiched between an antigen and a chromogenically-conjugated antibody. Below you will find a basic protocol for this assay. To coat the plate with the appropriate antigen, fill the microwells of a Nunc Maxi-Sorp
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