- 询价
- KLSW
- 美国 addgene
- KL-ZL-1099
- 2025年07月16日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20度
- 保质期：
2年
- 英文名：
pECFP-N1
- 库存：
100
- 供应商：
kelei-bio
- 规格：
20μl
启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞，荧光蛋白报告载体
质粒大小: 4733bp
原核抗性: Kan 筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R：GAAATTTGTGATGCTATTGC
质粒简介
pECFP-N1 encodes an enhanced cyan fluorescent variant of the Aequorea victoria green fluores-cent protein gene (GFP). The ECFP gene contains six amino acid substitutions. The Tyr-66 to Trpsubstitution gives ECFP fluorescence excitation (major peak at 433 nm and a minor peak at 453 nm)and emission (major peak at 475 nm and a minor peak at 501 nm) similar to other cyan emissionvariants. The other five substitutions (Phe-64 to Leu; Ser-65 to Thr; Asn-146 to Ile; Met-153to Thr; and Val-163 to Ala) enhance the brightness and solubility of the protein, primarily due toimproved protein folding properties and efficiency of chromophore formation. In addition to the chromophore mutations, ECFP contains >190 silent mutations that create an open reading framecomprised almost entirely of preferred human codons. Furthermore, upstream sequences flanking ECFP have been converted to a Kozak consensus translation initiation site. These changes increasethe translational efficiency of the ECFP mRNA and consequently the expression of ECFP in mammalian and plant cells.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验多克隆位点区 Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH. If you wish to digest
pEGFP-N1质粒图谱及信息 多克隆位点区 Restriction Map and Multiple Cloning Site of pEGFP-N1. (Unique restriction sites are in color or bold.) The Not I site follows the EGFP stop codon. The Xba I site (*) is methylated in the DNA provided by CLONTECH
【求助】SIN18-pRLL-hEFIap-EGFP-WRPE
gtwyx 慢病毒包装质粒SIN18-pRLL-hEFIap-EGFP-WRPE，求图谱。 gtwyx-fd@163.com希望得到各位战友的帮助！ freecell Write to author for it. http://www.nature.com/mt/journal/v3/n1/abs/mt200115a.html gtwyx 谢谢楼上的战友。我想知道国内有没有
技术资料暂无技术资料 索取技术资料
