基本信息
启动子: EF-1α core
复制子: pUC ori,SV40 ori
终止子: SV40 poly(A) signal
质粒分类: 病毒系列,慢病毒克隆载体
质粒大小: 7266bp
原核抗性: Amp
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物
质粒简介
Stable overexpression with cDNA lentivectors Strong and ubiquitous expression of the gene of interestSingle or double expression cassette with choice of reporter geneTarget gene expressed from CMV, EF1, or MSCV promoter Choose from FIV- or HIV-based vectors Gene expression from constitutive promoters,The multiple cloning site (MCS) located downstream of an internal promoter allows convenient cloning of your gene of interest.