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- 美国
- 2026年02月22日
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文献和实验Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction
Human NK-like cell lines are difficult to transfect using standard mammalian expression vectors and conventional transfection protocols, but they are susceptible to retroviral transduction as a means to introduce cDNAs. Our laboratory
We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human
that couple to similar signal transduction pathways. Genetic approaches to define NK cell receptor signaling pathways are hampered by the limited lifespan of cultured NK cells. We have therefore employed the immortalized human T cell line Jurkat and somatic
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