重要说明
本项服务所需细胞材料必须符合国家遗传资源相关法律规定。如有需要,三一造血可协助使用方取得伦理批件及申请人类遗传资源行政许可。
细胞分离服务所得细胞仅限用于科学研究使用,不得用于临床诊断及治疗,否则由此引发一切后果由使用方承担。
使用该产品引用文献 文献1.Repurposing screen identifies Amlodipine as an inducer of PD-L1 degradation and antitumor immunity
https://pubmed.ncbi.nlm.nih.gov/33323966/
文献2.Tubeimoside-1 induces TFEB-dependent lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting mTOR
https://pubmed.ncbi.nlm.nih.gov/34745852/
文献3.PTRF/Cavin-1 as a Novel RNABinding Protein Expedites the NF-kB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma
https://pubmed.ncbi.nlm.nih.gov/35069587/
T Cell Cytotoxicity Assay
Control and PTRF overexpressing TBD0220 cells were separately
transfected with siRNA for NEAT1 in DMEM/F12 medium.
Human peripheral blood mononuclear cells (PBMC; CBP-002,
StemEry) were maintained in DMEM/F12 medium. The PBMC
cells were activated with 100 ng/mL CD3 antibody (317325,
BioLegend), 100 ng/mL CD28 antibody (302933, BioLegend),
and 10 ng/mL IL2 (589102, BioLegend) for 24 h. Then, activated
PBMC cells were incubated with TBD0220 cells at 10:1 ratio in
the presence of caspase-3/7 green detection reagent (C10723,
Thermo Fisher, 1:1000) at 37°C for 12 hours according to the
manufacturer’s protocol. The cell nuclei were visualized by
staining with DAPI. All the images were observed using FV-
1200 laser scanning confocal microscope (Olympus
Corporation, Japan).