| 感受态细胞名称: | C43(DE3) Competent Cells |
|---|---|
| 菌株类型: | E.coli |
| 规格: | 10X110uL |
| 培养基: | LB培养基 |
| 生长条件: | 37 ℃, 有氧 |
| 基因型: | > |
| 抗性: | 无 |
| 质粒转化条件: | 42 ℃热激 |
| 应用: | 外源毒性蛋白表达 |
| 诱导方法: | IPTG |
| 菌株特点: | C43(DE3)能够高水平表达毒性蛋白,适用于T7启动子驱动的原核表达载体 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃
- 保质期:
6个月
- 英文名:
C43(DE3) OverExpress Competent Cells
- 库存:
大量现货
- 供应商:
信裕生物
- 规格:
10×100μl/50×100μl
C43(DE3) OverExpress Competent Cells感受态细胞基本信息
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
Transformation of plasmid DNA to competent E. coli cells Transformation is the process of getting the recombinant vector from a reaction mixture or vector solution into E. coli cells. To enable the cells to take up circular vector DNA
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