相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件:
常温
- 保质期:
三年
- 英文名:
pGene/v5-His B
- 库存:
60
- 供应商:
信裕生物
- 规格:
5ug质粒
基本信息
| 质粒类型: | 哺乳动物表达载体 |
|---|---|
| 启动子: | Gal4-E1b |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 4654bp (查看载体序列) |
| 5' 测序引物及序列: | pGene Fwd: 5'd[CTGCTATTCTGCTCAACCT]3' |
| 载体标签: | 6X His, V5 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Zeocin (博来霉素) |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1042 | pGene/v5-His B | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:
- Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
- Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料




