| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCS111 |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4146 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS111载体能够在多种细胞中进行高水平的瞬表达。pCS111载体同时也能进行体外转录和翻译。 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 2025年07月08日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCS111
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4146 bp ds-DNA circular SYN 29-JUL-2016
DEFINITION Vector that allows high-level transient expression in vertebrate
cells and in vitro transcription/translation.
ACCESSION DQ649434
KEYWORDS pCS111
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4146)
AUTHORS Biofeng Lab.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4146
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 79..156
/note="MCS1"
polyA_signal 187..321
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 390..425
/note="MCS2"
promoter complement(438..456)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature complement(479..481)
/note="ATG"
protein_bind 501..517
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(525..555)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(879..1467)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1638..2498)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2499..2603)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(2629..3084)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3162..4146
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcccgggaat ctggccgcgg
121 ccgcaaggcc tgatatctct agaccgcggc tcgagcctct cgccctatag tgagtcgtat
181 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
361 ggggaggtgt gggaggtttt ttaattcgcg gcgcgccgcg gcgccaatgc attgggcccg
421 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
1321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
2641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
2701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
2761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
2821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
2881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
2941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
3001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
3061 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
3121 ggcgatcggt gcgggcctct tcgctattac gccagtcgat cgaccatagc caattcaata
3181 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata
3241 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata
3301 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga
3361 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc
3421 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat
3481 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta
3541 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc
3601 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca
3661 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg
3721 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct
3781 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca
3841 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac
3901 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaagtac
3961 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc
4021 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca
4081 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta
4141 gggaac
//
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pCS111体
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