| 出品人: | ZYbscience |
|---|---|
| 载体名称: | pBTB-2 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 3740 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pBTB-2
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS DQ058730 3740 bp DNA circular SYN 06-FEB-2009
DEFINITION Broad host range vector pBTB-2, complete sequence.
ACCESSION DQ058730
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
REFERENCE 3 (bases 1 to 3740)
AUTHORS Godiska,R.
TITLE Direct Submission
JOURNAL Submitted (06-FEB-2009) Lucigen Corp., 2120 W. Greenview Dr., #9,
Middleton, WI 53562, USA
REMARK Sequence update by submitter
COMMENT On Feb 6, 2009 this sequence version replaced gi:69048318.
FEATURES Location/Qualifiers
source 1..3740
/organism="Broad host range vector pBTB-2"
/mol_type="other DNA"
/db_xref="taxon:334962"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99720.1"
/db_xref="GI:69048319"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..2446
/gene="kan"
/note="kanamycin resistance gene from TN903"
CDS 1631..2446
/gene="kan"
/codon_start=1
/transl_table=11
/product="kanamycin kinase"
/protein_id="AAY99721.1"
/db_xref="GI:69048320"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
regulatory 2463..2494
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(2522..3451)
/gene="araC"
CDS complement(2522..3451)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99722.1"
/db_xref="GI:69048321"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 3459..3726
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga
1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct
2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt
2521 tttatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
2581 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
2641 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
2701 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
2761 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
2821 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg
2881 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
2941 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
3001 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa
3061 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
3121 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga
3181 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct
3241 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
3301 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
3361 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
3421 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
3481 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
3541 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
3601 acattgatta tttgcacggc gtcacacttt gctatgccat aacattttta tccataagat
3661 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
3721 tttgggctag agaattcgtc
//
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pBTB-2载体
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