PX395载体

PX395载体

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  • ¥1000
  • ZYbscience
  • 中国/美国
  • ZY68332
  • 2025年07月12日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      PX395

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: PX395 或 PX395 Roseburia intestinalis Cas9
    质粒类型: CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
    高拷贝/低拷贝: 高拷贝
    插入位点: 限制性内切酶，多克隆位点
    启动子: CMV
    载体大小: 10616 bp
    5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
    载体标签: NLS (C ter)；HA、NLS (N ter)
    载体抗性: 氨苄青霉素
    筛选标记: Neomycin
    克隆菌株: Stbl3
    宿主细胞（系）: 昆虫细胞
    备注: --
    产品目录号: ZY68332
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 杆状病毒

    载体质粒图谱和多克隆位点信息

    PX395载体图谱


    载体简介

    载体序列

    LOCUS       PX395 Roseburia intestinalis Cas9	10616 bp 	DNA	SYN
DEFINITION  PX395 Roseburia intestinalis Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="PX395 Roseburia intestinalis Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5307
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL
                     DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR
                     RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL
                     HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS
                     WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT
                     SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK
                     INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK
                     AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY
                     KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE
                     AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI
                     NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ
                     QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE
                     YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY
                     NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC
                     CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE
                     TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD
                     TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA
                     KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG
                     KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP
                     IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL
                     GHVSVVRSEKRMGFS*"
     CDS             complement(1999..3270)
                     /label="ORF frame 3"
                     /translation="MLEFGRSISQQAFHLDGHQSQLGQSFGAGIVIFVTSQNHSDLIK
                     QVPDFVLGQILHSLFQAFLVIVIVWHPDKLGFFGILVLLLLLFFSSGLLVVQVVEQDV
                     LRQAQFLADCSVLHLFLGKQQQSQGSPLFLSEIPCLAVLIDQLCVVDFLQSHEIFGCV
                     GPLSAPQLSLIPQKSTFPHSVREALHCIRGGLPIWLHSRPQVKRALGGHDLQVLFLQF
                     LSGFFVAQSALGAENLLDFLHQHCPQHCVLIIFSLVAGGQDVPFLTPRAASPEGLVQV
                     DHLAHCPVSLFLHSFLILVCGLQHCPVFLGCCLGFPGASEAPMLSDVQQHLGQFLIGQ
                     FSVQSLIPQLVHIWQSGHVVSLQKIVNIDQPLVLEQPLDSVQAVFPSPQSAPCGSSCS
                     PGSCQAFACLWDLCGIENAHSQVDNPHRLIV*"
     CDS             complement(4276..4764)
                     /label="ORF frame 3"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(5340..5357)
                     /label="Sp6_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    5362..5386
                     /label="attB2"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     terminator      5398..5864
                     /label="hGH_PA_terminator"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     terminator      5870..5905
                     /label="T7_terminator"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     rep_origin      6022..6328
                     /label="f1_origin"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(6442..6462)
                     /label="pBABE_3_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    complement(6448..6663)
                     /label="SV40_enhancer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     promoter        6460..6728
                     /label="SV40_promoter"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     rep_origin      6627..6704
                     /label="SV40_origin"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     misc_feature    6689..6708
                     /label="SV40pro_F_primer"
                     /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF
                     QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH
                     IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC
                     SL*"
     CDS             6843..7637
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6846..7634
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7152..7688)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7814..7933
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7902..7921
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8124..8159
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8182..8199)

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