LOCUS PX395 Roseburia intestinalis Cas9 10616 bp DNA SYNDEFINITION PX395 Roseburia intestinalis Cas9ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..10616 /organism="PX395 Roseburia intestinalis Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5307 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMRENGSDERRRNMDEKMDYRIGL DIGIASVGWAVLQNNSDDEPVRIVDLGVRIFDTAEIPKTGESLAGPRRAARTTRRRLR RRKHRLDRIKWLFENQGLINIDDFLKRYNMAGLPDVYQLRYEALDRKLTDEELAQVLL HIAKHRGFRSTRKAETAAKENGAVLKATDENQKRMQEKGYRTVGEMIYLDEAFRTGCS WSEKGYILTPRNKAENYQHTMLRAMLVEEVKEIFSSQRRLGNEKATEELEEKYLEIMT SQRSFDLGPGMQPDGKPSPYAMEGFSDRVGKCTFLGDQGELRGAKGTYTAEYFVALQK INHTKLVNQDGETRNFTEEERRALTLLLFTQKEVKYAAVRKKLGLPEDILFYNLNYKK AATKEEQQKENQNTEKAKFIGMPYYHDYKKCLEERVKYLTENEVRDLFDEIGMILTCY KNDDSRTERLAKLGLVPIEMEGLLAYTPTKFQHLSMKAMRNIIPFLEKGMTYDKACEE AGYDFKADSKGTKQKLLTGENVNQTINEITNPVVKRSVSQTVKVINAIIRTYGSPQAI NIELAREMSKTFEERRKIKGDMEKRQKNNEDVKKQIQELGKLSPTGQDILKYRLWQEQ QGICMYSGKTIPLEELFKPGYDIDHILPYSITFDDSFRNKVLVTSQENRQKGNRTPYE YMGNDEQRWNEFETRVKTTIRDYKKQQKLLKKHFSEEERSEFKERNLTDTKYITTVIY NMIRQNLEMAPLNRPEKKKQVRAVNGAITAYLRKRWGLPQKNRETDTHHAMDAVVIAC CTDGMIQKISRYTKVRERCYSKGTEFVDAETGEIFRPEDYSRAEWDEIFGVHIPKPWE TFRAELDVRMGDDPKGFLDTHSDVALELDYPEYIYENLRPIFVSRMPNHKVTGAAHAD TIRSPRHFKDEGIVLTKTALTDLKLDKDGEIDGYYNPQSDLLLYEALKKQLLLYGNDA KKAFAQDFHKPKADGTEGPVVRKVKIQKKQTMGVFVDSGNGIAENGGMVRIDVFRVNG KYYFVPVYTADVVKKVLPNRASTAHKPYGEWKVMEDKDFLFSLYSRDLIHIKSKKDIP IKMVNGGMEGIKETYAYYIGADISAANIQGIAHDSRYKFRGLGIQSLDVLEKCQIDVL GHVSVVRSEKRMGFS*" CDS complement(1999..3270) /label="ORF frame 3" /translation="MLEFGRSISQQAFHLDGHQSQLGQSFGAGIVIFVTSQNHSDLIK QVPDFVLGQILHSLFQAFLVIVIVWHPDKLGFFGILVLLLLLFFSSGLLVVQVVEQDV LRQAQFLADCSVLHLFLGKQQQSQGSPLFLSEIPCLAVLIDQLCVVDFLQSHEIFGCV GPLSAPQLSLIPQKSTFPHSVREALHCIRGGLPIWLHSRPQVKRALGGHDLQVLFLQF LSGFFVAQSALGAENLLDFLHQHCPQHCVLIIFSLVAGGQDVPFLTPRAASPEGLVQV DHLAHCPVSLFLHSFLILVCGLQHCPVFLGCCLGFPGASEAPMLSDVQQHLGQFLIGQ FSVQSLIPQLVHIWQSGHVVSLQKIVNIDQPLVLEQPLDSVQAVFPSPQSAPCGSSCS PGSCQAFACLWDLCGIENAHSQVDNPHRLIV*" CDS complement(4276..4764) /label="ORF frame 3" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(5340..5357) /label="Sp6_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature 5362..5386 /label="attB2" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" terminator 5398..5864 /label="hGH_PA_terminator" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" terminator 5870..5905 /label="T7_terminator" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" rep_origin 6022..6328 /label="f1_origin" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(6442..6462) /label="pBABE_3_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature complement(6448..6663) /label="SV40_enhancer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" promoter 6460..6728 /label="SV40_promoter" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" rep_origin 6627..6704 /label="SV40_origin" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" misc_feature 6689..6708 /label="SV40pro_F_primer" /translation="MKVLSEGLFGIIAIQQQLLFQCFVQQQIGLGVIVSVDLPILVQF QVGQCGLSQHDALIFKVSWGPNCVSMGCSGDLVIWHSAHEDRPQVFVDVLGIIQLQSH IAMSVQESLWIVPHSDVQFGAKCLPGLWDVHAENFIPLGPAVVLGSKDFASLCIDELC SL*" CDS 6843..7637 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6846..7634 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7152..7688) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7814..7933 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7902..7921 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8124..8159 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8182..8199)