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pcambia1304
20
泽叶生物
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA1304 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 35S |
| 载体大小: | 12362 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13 Forward：TGTAAAACGACGGCCAGT |
| 载体标签: | mGFP5、GUS |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 农杆菌/植物细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
LOCUS AF234300 12361 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1304, complete sequence.
ACCESSION AF234300
VERSION AF234300.1 GI:7638083
KEYWORDS .
SOURCE Binary vector pCAMBIA-1304
ORGANISM Binary vector pCAMBIA-1304
other sequences; artificial sequences; vectors.
REFERENCE 1 (sites)
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
for plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 12361)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REMARK Full description of constructs
REFERENCE 3 (bases 1 to 12361)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..12361
/organism="Binary vector pCAMBIA-1304"
/mol_type="other DNA"
/db_xref="taxon:118392"
CDS 2..2569
/note="mgfp5*-gusA (N358Q) fusion (2-730/731-2569)"
/codon_start=1
/product="mGFP5-GUSA fusion protein"
/protein_id="AAF65352.1"
/db_xref="GI:7638085"
/translation="MVDLTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
KLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
QKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK
RDHMVLLEFVTAAGITHGMDELYKASLRPVETPTREIKKLDGLWAFSLDRENCGIDQR
WWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAV
THYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVI
TDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVV
ANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIY
PLRVGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGA
NSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVN
GETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTR
PITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLH
QPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFA
TSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
misc_feature complement(1802..1804)
/note="N358Q glycosylation mutation"
misc_feature 2598..2850
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
misc_feature 2888..2913
/note="right border T-DNA repeat"
misc_feature complement(3954..4954)
/note="STA region from pVS1 plasmid"
rep_origin complement(5547..6547)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(6957..7217)
/note="bom site from pBR322"
rep_origin complement(7357..7637)
/note="pBR322 origin of replication"
CDS complement(7928..8722)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65353.1"
/db_xref="GI:7638086"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9147..9172
/note="left border repeat from C58 T-DNA"
misc_feature 9239..9447
/note="CaMV 3'UTR (polyA signal)"
CDS complement(9463..10488)
/note="hptII (hygromycin resistance) gene"
/codon_start=1
/product="hygromycin phosphotransferase"
/protein_id="AAF65354.1"
/db_xref="GI:7638087"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK"
promoter complement(10524..11331)
/note="CaMV35S2; CaMV 35S promoter, duplicated"
promoter 11452..11506
/note="PlacZ; lacZ promoter"
CDS 11534..11767
/codon_start=1
/product="LacZ alpha fragment"
/protein_id="AAF65351.1"
/db_xref="GI:7638084"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 11576..11626
/note="pUC18 MCS; polylinker"
promoter 11818..12355
/note="CaMV35S; 35S promoter from CaMV"
ORIGIN
1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
721 atacaaagct agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
781 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
841 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
901 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
961 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
1021 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
1081 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
1141 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
1201 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
1261 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
1321 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
1381 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
1441 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
1501 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
1561 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
1621 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
1681 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
1741 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
1801 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
1861 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
1921 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
1981 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
2041 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
2101 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
2161 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
2221 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
2281 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
2341 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
2401 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
2461 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
2521 gcagcaggga ggcaaacaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca
2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt
7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg
9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc
10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac
10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg
10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
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