| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA1305.1 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Lac |
| 载体大小: | 11847 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素（Hygromycin）、GUS |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 询价记录
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pcambia1305.1
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AF354045 11846 bp DNA circular SYN 16-APR-2001
DEFINITION Binary vector pCAMBIA-1305.1, complete sequence.
ACCESSION AF354045
VERSION AF354045.1 GI:13540346
KEYWORDS .
SOURCE Binary vector pCAMBIA-1305.1
ORGANISM Binary vector pCAMBIA-1305.1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 11846)
AUTHORS Jefferson,R.A., Kilian,A. and Keese,P.K.
TITLE Microbial genes for secreted beta-glucuronidases, gene products and
uses thereof
JOURNAL Patent: PCT (PCT/US98/19217)-A 09-SEP-1998;
CAMBIA; Molecular Technologies; Canberra;
Australia;
REFERENCE 2 (bases 1 to 11846)
AUTHORS Nguyen,T.A. and Jefferson,R.A.
TITLE Binary vector pCAMBIA-1305.1
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 11846)
AUTHORS Nguyen,T.A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2001) Molecular Technologies, CAMBIA, GPO Box
3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..11846
/organism="Binary vector pCAMBIA-1305.1"
/mol_type="genomic DNA"
/db_xref="taxon:156490"
CDS join(2..16,207..2054)
/note="GUSPlus-His6"
/codon_start=1
/transl_table=11
/product="beta-glucuronidase"
/protein_id="AAK29426.1"
/db_xref="GI:13540350"
/translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYE
SKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHK
AIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHE
EGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQ
GKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTID
VYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWI
GANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEK
IRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQK
RPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRC
PGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFAD
FATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"
intron 17..206
/note="catalase intron"
polyA_signal 2083..2335
/note="poly(A) signal from nopaline synthase"
misc_feature 2354..2378
/note="T-DNA right border"
misc_feature 3439..4439
/note="sta region from pVS1 plasmid"
rep_origin 5032..6032
/note="pVS1 rep"
misc_feature 6442..6702
/note="pBR322 bom site"
rep_origin 6842..7122
/note="pBR322 ori"
CDS complement(7413..8207)
/note="kanamycin resistance gene"
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="AAK29424.1"
/db_xref="GI:13540348"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8632..8657
/note="T-DNA left border"
polyA_site 8798..8805
CDS complement(8948..9973)
/note="hygromycin resistance gene"
/codon_start=1
/transl_table=11
/product="hygromycin phosphotransferase"
/protein_id="AAK29425.1"
/db_xref="GI:13540349"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK"
promoter complement(10009..10816)
/note="CaMV35Sx2; CaMV35S eukaryotic promoter with
duplicated enhancer region"
promoter 10938..11018
/note="PlacZ prokaryotic promoter"
CDS 11019..11252
/note="beta-galactosidase alpha fragment"
/codon_start=1
/transl_table=11
/product="lacZ alpha fragment"
/protein_id="AAK29423.1"
/db_xref="GI:13540347"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 11033..11089
/note="pUC18 polylinker multiple cloning site"
promoter 11303..11840
/note="CaMV35S eukaryotic promoter"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
4741 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
4801 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
4861 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
4921 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
4981 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
5041 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
5101 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
5161 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
5221 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
5281 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
5341 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
5401 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
5461 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
5521 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
5581 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
5641 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
5701 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
5761 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
5821 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
5881 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
5941 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
6001 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
6061 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
6121 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
6181 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
6241 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
6421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
6481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7381 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
7441 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
7501 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
7561 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
7621 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
7681 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
7741 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
7801 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
7861 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
7921 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
7981 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
8041 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
8101 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
8161 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
8221 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
8281 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
8341 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
8401 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
8461 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
8521 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
8581 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
8641 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
8701 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
8761 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
8821 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
8881 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
8941 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
9001 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
9061 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
9121 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
9181 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
9241 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
9301 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
9361 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
9421 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
9481 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
9541 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
9601 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
9661 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
9721 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
9781 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
9841 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
9901 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
9961 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
10021 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
10081 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
10141 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
10201 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
10261 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
10321 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
10381 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
10441 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
10501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
10561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
10621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
10681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
10741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
10801 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
10861 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
10921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
10981 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga
11041 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
11101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
11161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
11221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
11281 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
11341 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
11401 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
11461 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
11521 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
11581 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
11641 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
11701 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
11761 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
11821 tggagagaac acgggggact cttgac
//
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