LOCUS pCAMBIA1105.1 12137 bp ds-DNA circular SYN 25-JUL-2016DEFINITION Agrobacterium binary vector for plant transformation, with hygromycin- and spectinomycin-resistance and GUSPlus(TM) genes plus the pUC19 MCS.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 12137) AUTHORS Cambia TITLE Direct Submission JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 4740.COMMENT VNTDATE|658713600|COMMENT VNTDBDATE|776304000|COMMENT VNTNAME|pCAMBIA1105.1|COMMENT VNTAUTHORNAME|Cambia|FEATURES Location/Qualifiers CDS join(2..16,207..2024) /codon_start=1 /product="beta-glucuronidase" /label=GUSPlus(TM) /note="codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria" intron 17..206 /label=cat1 intron /note="castor bean catalase intron, modified" CDS 2031..2048 /codon_start=1 /product="6xHis affinity tag" /label=6xHis terminator 2080..2332 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 2354..2378 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 3678..4307 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 4736..5809 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 5875..6069 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 6413..6553 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(6739..7327) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7571..8362) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase 氨基糖苷类抗生素" /label=SmR /note="confers resistance to spectinomycin and streptomycin" misc_feature 8923..8947 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 9025..9199 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(9239..10264) /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" promoter complement(10332..11009) /label=35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 11236..11266 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11274..11290 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11298..11314 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 11310..11543 /codon_start=1 /gene="lacZ (truncated)" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha misc_feature 11324..11380 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(11384..11400) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 11777..12122 /label=35S promoter /note="strong constitutive promoter from cauliflower mosaic virus CaMV 35S promoter"ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg 241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag 301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa 361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt 421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg 481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg 541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg 601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg 661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca 721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg 781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact 841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg 901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac 961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg 1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca 1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc 1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca 1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg 1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca 1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg 1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg 1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca 1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc 1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc 1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc 1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg 1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg 1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg 1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc 1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct 1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc 2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa 2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta 2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt 2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc 2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta 2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa 2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg 2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa 2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac 2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac 2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga 2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat 2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga 3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga 3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt 3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat 3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac 3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg 3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg 3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc 3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc 3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg 3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt 3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa 3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca 3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt 3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac 3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc 3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga 3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca 4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag 4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg 4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc 4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc 4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa 4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag 4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca 4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat 4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa 4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca 4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct 4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag 4741 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 4801 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 4861 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 4921 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 4981 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 5041 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 5101 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 5161 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 5221 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 5281 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 5341 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 5401 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 5461 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 5521 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 5581 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 5641 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 5701 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 5761 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 5821 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 5881 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 5941 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 6001 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 6061 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 6121 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 6181 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 6241 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 6301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 6361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 6421 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 6481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 6541 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7381 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc 7441 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc 7501 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc 7561 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt 7621 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc 7681 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag 7741 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa 7801 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt 7861 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg 7921 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt 7981 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt 8041 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga 8101 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt 8161 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa 8221 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca 8281 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt 8341 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg