pCAMBIA0105.1R载体

LOCUS       pCAMBIA0105.1R         10033 bp ds-DNA     circular SYN 25-JUL-2016
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            spectinomycin-resistance and GUSPlus(TM) genes.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Cambia
  TITLE     Direct Submission
  JOURNAL   Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 4740.
COMMENT     VNTDATE|658800000|
COMMENT     VNTDBDATE|776304000|
COMMENT     VNTNAME|pCAMBIA0105.1R|
COMMENT     VNTAUTHORNAME|Cambia|
FEATURES             Location/Qualifiers
     CDS             join(2..16,207..2024)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2031..2048
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     terminator      2080..2332
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2354..2378
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3678..4307
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             4736..5809
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5875..6069
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6413..6553
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6739..7327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7571..8362)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase氨基糖苷类抗生素"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
     misc_feature    8923..8947
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        9051..9081
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9089..9105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9113..9129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9125..9439
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
     misc_feature    9142..9247
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(9255..9273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9280..9296)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9673..10018
                     /label=35S promoter
                     /note="CaMV 35S promoter。strong constitutive promoter from 
                     cauliflower mosaic virus"
ORIGIN
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
      241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag
      301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
      361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt
      421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg
      481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
      541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg
      601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
      661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
      721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
      781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
      841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
      901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac
      961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
     1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca
     1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc
     1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca
     1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg
     1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
     1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
     1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
     1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
     1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc
     1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
     1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc
     1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg
     1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
     1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
     1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
     1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct
     1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
     2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     4741 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     4801 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     4861 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     4921 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     4981 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     5041 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     5101 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     5161 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     5221 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     5281 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     5341 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     5401 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     5461 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     5521 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     5581 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     5641 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     5701 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     5761 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     5821 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     5881 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     5941 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     6001 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     6061 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     6121 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     6181 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     6241 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     6301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     6421 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     6481 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6541 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6601 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6661 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6721 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6781 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6841 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6901 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7081 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7141 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7201 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7261 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7321 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7381 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
     7441 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     7501 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
     7561 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     7621 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     7681 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     7741 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     7801 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     7861 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     7921 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     7981 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     8041 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     8101 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     8161 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     8221 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     8281 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     8341 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
     8401 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     8461 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa
     8521 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac
     8581 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggcttatgt ccactgggtt
     8641 cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcc gcggtgatca caggcagcaa
     8701 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     8761 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     8821 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     8881 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     8941 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
     9001 aattaacgcc gaattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     9061 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     9121 agctatgacc atgattacgc caagcttggt accgagctcg gatccactag taacggccgc
     9181 cagtgtgctg gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag
     9241 agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     9301 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     9361 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9421 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca
     9481 gtttagcttc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
     9541 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
     9601 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     9661 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
     9721 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
     9781 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
     9841 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     9901 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     9961 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
    10021 ggggactctt gac
//