企业认证
-20℃低温保存
三年
pPZP111
20
泽叶生物
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pPZP111 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 8909 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | 无 |
| 载体抗性: | chloramphenicol、Kanamycin |
| 筛选标记: | Neomycin |
| 克隆菌株: | HB101 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | 重组质粒可用蓝白斑法筛选 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
LOCUS pPZP111 8909 bp DNA SYN
DEFINITION pPZP111
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8909
/organism="pPZP111"
/mol_type="other DNA"
CDS 202..888
/label="ORF frame 1"
/translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG
TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA
DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR
ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA
AENERQEEQA*"
CDS 1187..1816
/label="ORF frame 2"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
CDS complement(2091..2684)
/label="ORF frame 1"
/translation="MGNRHQYQVVIPHTGHAGRPCGNLYVPVWKLVADHLASSSVTLR
QTENGHVHDAATIAGAHVIEHRNEKIWLLVALGRLPNRRRTGCRRLPGFFADSISGRL
PRFTGACFCLDALPLGGLRGLQLLHQVITQRRADLYRAGWFATVTPIPRAWGFQCHCR
AGRQPSRLRLANRPFLHTWGIPRRRCLVVLDFPCRLL*"
CDS 2211..3317
/label="ORF frame 3"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
misc_feature complement(3813..3835)
/label="pGEX_3_primer"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
rep_origin complement(4232..4851)
/label="pBR322_origin"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
gene complement(5106..5765)
/label="CAT/CamR"
/gene="CAT/CamR"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
CDS complement(5106..5906)
/label="ORF frame 1"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKF
YPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDF
RQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVF
TMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
CDS complement(6643..7440)
/label="ORF frame 3"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene complement(6646..7425)
/label="NeoR/KanR (variant)"
/gene="NeoR/KanR (variant)"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(7932..8390)
/label="ORF frame 1"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
promoter 8405..8434
/label="lac_promoter"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
misc_feature 8448..8470
/label="M13_pUC_rev_primer"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
promoter 8469..8487
/label="M13_reverse_primer"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
promoter complement(8553..8569)
/label="M13_forward20_primer"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
misc_feature complement(8562..8584)
/label="M13_pUC_fwd_primer"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
misc_feature 8562..8705
/label="lacZ_a"
/translation="MSELTHINCVALTARFPVGKPVVPAALMNRPTRGERRFAYWLEQ
LANMVEHDTLVYSKNIKDTVSEDQRAIETFQQRVISGNLLGFHCPAICHFIKRTVEKE
GGTYKCHHCDKGKAIVQDASADSGPKDGPPPTRSIVEKEDVPTTSSKQVD*"
ORIGIN
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