pLVX-TRE3G-mCherry载体

LOCUS       pLVX-TRE3G-mCherry  9115 bp    DNA   circular             9-MAY-2012
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     631360, 631349, 631352, 631255
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=psi
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                       /label=cPPT/CTS
     misc_feature    3896..4399
                     /label=pPGK
     misc_feature    4420..5019
                     /label=Puro
     misc_feature    5037..5628
                     /label=WPRE
     3'clip          5832..6468
                     /label=3'\LTR
     misc_feature    complement(6938..7608)
                     /label=pUC\ori
     misc_feature    2564..3274
                     /label=mCherry
     misc_feature    3283..3871
                     /label=IRES
     misc_feature    3872..3895
                     /label=MCS
     misc_feature    2209..2560
                     /label=pTRE3GV
     misc_feature    complement(8614..8718)
                     /label=pAmp(R)
     misc_feature    complement(7753..8613)
                     /label=Amp(R)
BASE COUNT     2273 a      2340 c      2410 g      2092 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggtga gcaagggcga 
     2581 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 
     2641 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 
     2701 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 
     2761 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 
     2821 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 
     2881 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 
     2941 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 
     3001 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 
     3061 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 
     3121 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 
     3181 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 
     3241 ctccaccggc ggcatggacg agctgtacaa gtagggccgc cccggccggc ccctctccct 
     3301 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 
     3361 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 
     3421 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 
     3481 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 
     3541 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 
     3601 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 
     3661 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 
     3721 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt 
     3781 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 
     3841 ttcctttgaa aaacacgatg ataatatggc ccccgggacg cgtcatatgg aattctaccg 
     3901 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 
     3961 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 
     4021 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 
     4081 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 
     4141 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 
     4201 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 
     4261 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 
     4321 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 
     4381 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 
     4441 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 
     4501 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 
     4561 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 
     4621 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 
     4681 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 
     4741 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 
     4801 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 
     4861 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 
     4921 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 
     4981 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac ggggcgcgtc tggaacaatc 
     5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 
     5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 
     5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 
     5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 
     5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 
     5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     5401 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 
     5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 
     5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 
     5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg 
     5641 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 
     5701 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca gtcacacctc 
     5761 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
     5821 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 
     5881 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 
     5941 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 
     6001 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 
     6061 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 
     6121 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 
     6181 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 
     6241 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 
     6301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     6361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     6421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     6481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 
     6541 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     6601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     6661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     6721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     6781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     7501 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac 
     7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 
     8761 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     8821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     8881 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 
     8941 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt 
     9001 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 
     9061 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt