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- 详细信息
- 技术资料
- 保存条件:
常温
- 保质期:
三年
- 英文名:
FUGW
- 库存:
60
- 供应商:
信裕生物
- 规格:
5ug质粒
基本信息
| 质粒类型: | 慢病毒载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | hUbc |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 9941 bp (查看载体序列) |
| 5' 测序引物及序列: | hUBCpro-F: TGAAGCTCCGGTTTTGAACT |
| 3' 测序引物及序列: | EGFP-N: CGTCGCCGTCCAGCTCGACCAG |
| 载体标签: | C-EGFP |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: | 载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率 |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1997 | FUGW | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.
Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.
Please note that there are 2 gaps upstream of the EGFP between author's provided sequence and Addgene's quality control sequence. These gaps are in the non-coding vector region and should not affect the expression of EGFP.
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