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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C for one year from date of receipt. Avoid repeated freeze-thaw cycles.. Shipped in room temperature.
- 保质期:
12 months from date of receipt.
- 英文名:
Antibody diluent buffer
- 库存:
99
- 供应商:
Biorbyt
- 规格:
12 ml
描述: Antibody diluent buffer
保存条件: Store at -20°C for one year from date of receipt. Avoid repeated freeze-thaw cycles.. Shipped in room temperature.
备注: For research use only.
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文献和实验Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
免疫组化技术-Immunohistochemical Protocols
2) or a biotin conjugated (Table2) secondary antibody is used, both at a dilution of 1:500 in BSA diluent for 1 hr, Wash in PBS 3x5 min Incubate in avidin-biotin-peroxidase complex (ABC reagent
should be at ambient temperature prior to use. PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water. Wash Buffer: 0.05% Tween-20 in PBS Block Buffer: 1% BSA in PBS* Diluent: 0.05% Tween-20, 0.1% BSA in PBS* *Sterile filter and store at 4°C
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