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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
- 保质期:
6个月
- 英文名:
Vaccinia Virus B18R Protein, His Tag
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
50 ug
应用笔记:This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.3 kDa. The protein migrates as 50-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
反应种属:Virus
纯度:90%
分子量:40.3 kDa
表达区域:His 20 - Glu 351
内毒素:1.0 EU per μg
标签:C-10×His
防腐剂:PBS, pH 7.4
形态:Powder
标记物:Unconjugated
Uniprot ID:P25213
Note:For research use only.


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文献和实验Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination
Vaccinia virus, the prototype Orthopoxvirus, is widely used in the laboratory as a model system to study various aspects of viral biology, virus-host interactions, and as a protein expression system and a vaccine vector. The ubiquitous use
Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
/El, E1/E2, E2/p7, and p7/NS2 sites) (9 ,13 ), whereas an HCV-encoded serine proteinase located in the N-termmal one-third of the NS3 protein is responsible for four cleavages in the NS region (3/4A, 4A/4B, 4B/5A, and 5A/5B sites) (11 ,14 –16 ).
Imaging of Vaccinia Virus Entry into HeLa Cells
. The methods presented here describe how to construct a fluorescent recombinant vaccinia virus expressing the core protein A4L fused to mCherry, and how to detect the virus movement on actin-EYFP-expressed HeLa cells.
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