Lhc1

Lhc1

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  • ¥4845
  • Agrisera
  • AS08 282a
  • 美国
  • 2025年07月14日
  • western blot (WB)
  • 植物
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    • 详细信息
    • 技术资料
    • 抗体名

      兔抗光系统I捕光天线全复合体多克隆抗体

    • 抗体英文名

      Lhc1|PS 1 light harvesting holo-complex

    • 浓度

      见说明书

    • 应用范围

      western blot (WB)

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单行

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      200 µl

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


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    Lhc1|PS 1 light harvesting holo-complex介绍:

    货 号:AS08 282

    中文名称:兔抗光系统I捕光天线全复合体多克隆抗体

    英文名称:Lhc1|PS 1 light harvesting holo-complex

    应用:western blot (WB)

    规格:200 µl

    价格:4845元

    Lhc1|PS 1 light harvesting holo-complex简介:

    PRODUCT INFORMATION
    background

    The light-harvesting system of higher plant is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

    immunogen

    purified LHC complex from Porphyridium cruentum

    host

    rabbit

    clonality

    polyclonal

    purity

    serum

    format

    lyophilized

    quantity 200 µl
    reconstitution For reconstitution add 200 µl of sterile water.
    storage

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB)

    related products

    AS09 407 | anti-Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas

    AS09 408 | anti-Lhcbm5 | chlorophyll a-b binding protein of LHCII

    AS06 117 | anti-Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

    additional information

    to be added when available

    APPLICATION INFORMATION
    recommended dilution

    1: 1000 with standard ECL (WB)

    expected | apparent MW

    20-25 kDa

    confirmed reactivity

    Aureococcus anophagefferens,Guillardia theta,Heterosigma akashiwo, Thalassiosira pseudonana,Porphyridium cruentum

    predicted reactivity

    diatoms

    not reactive in

    no confirmed exceptions from predicted reactivity known in the moment

    additional information

    strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum, reactivity to a few Lhc2 polypeptides of spinach is very low

    selected references

    Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1.
    Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566

    application example

    2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana,(2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and
    probed with anti-Lhc1 (AS08 282, 1:6000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated, Bio-rad) in PBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in PBS-T (15, +5, +5, +5 min). All steps were
    performed at RT with agitation. Signal was detected with standard ECL. Exposure time was 2 min.


    Courtesy of Meriem Alami and Beverley Green.

    western blot using anti-Lhc1 antibodies

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