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兔抗拟南芥质膜水孔蛋白PIP2;1+PIP2;2多克隆抗体
PIP2;1+PIP2;2|aquaporin PIP2;1+PIP2;2
见说明书
ELISA (ELISA), western blot (WB)
兔
植物
无
多抗/单行
低温
IgG
100 µl
供应商:上海经科化学科技有限公司
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PIP2;1+PIP2;2|aquaporin PIP2;1+PIP2;2介绍:
货 号:AS09 488
中文名称:兔抗拟南芥质膜水孔蛋白PIP2;1+PIP2;2多克隆抗体
英文名称:PIP2;1+PIP2;2|aquaporin PIP2;1+PIP2;2
应用:ELISA (ELISA), western blot (WB)
规格:100 µl
价格:6600元
PIP2;1+PIP2;2|aquaporin PIP2;1+PIP2;2简介:
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APPLICATION INFORMATION | ||
recommended dilution | 1: 8000 (ELISA), 1: 1000 with standard ECL (WB) |
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expected | apparent MW | 30.34 | 28 kDa (Raphanus sativus) |
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confirmed reactivity | Arabidopsis thaliana, Oryza sativa, Raphanus sativus |
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predicted reactivity | dicots including: Brassica napus, Gossypium mexicanum, |
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not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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additional information | Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Peptide used to elicit this antibody is also conserved in Arabidopsis thaliana PIP2;1,PIP2;2, PIP2;3 and this antibody reacted also with PIP2;2 in experiments with knock out plants. Manufactured by Operon Biotechnology |
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selected references | Markus G. et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405. |
application example
1 µg and 10 µg of crude membrane fraction/lane fromArabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;1 antibodies (AS09 488, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. |
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