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兔抗拟南芥光系统II PsbS蛋白多克隆抗体
PsbS|22 kDa Lhc-like PSII protein
见说明书
western blot (WB)
兔
植物
无
多抗/单行
低温
IgG
200 µl
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兔抗拟南芥光系统II PsbS蛋白多克隆抗体介绍:
货 号:AS09 533
中文名称:兔抗拟南芥光系统II PsbS蛋白多克隆抗体
英文名称:PsbS|22 kDa Lhc-like PSII protein
应用:western blot (WB)
规格:200 µl
价格:3975元
兔抗拟南芥光系统II PsbS蛋白多克隆抗体简介:
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APPLICATION INFORMATION | ||
recommended dilution | 1 : 2000 with standar ECL (WB) |
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expected | apparent MW | 28 | 22 kDa forArabidopsis thaliana |
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confirmed reactivity | Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Picea abies, Ricinus communis, Spinacia oleracea, Zea mays | |
predicted reactivity | dicots including: Cucumis sativus, Solanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, trees: Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii |
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not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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additional information | to be added when available |
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selected references | Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009. Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Belgio et al. (2015). Light harvesting superstructures of green plant chloroplasts lacking photosystems. Plant Cell Environ. 2015 Mar 4. doi: 10.1111/pce.12528. Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16. Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16. Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.
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application example 1 5 µg of total extract from (1) Arabidopsis thaliana leaf, (2) Spinacia oleracea (3)Hordeum vulgare (4) Zea mays extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds. |
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