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V5 Antibody

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  • ¥3975
  • Agrisera
  • AS15 3027a
  • 美国
  • 2025年07月14日
  • west blot
  • 植物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      鼠抗V5标签抗体

    • 抗体英文名

      V5

    • 浓度

      见说明书

    • 应用范围

      west blot

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单抗

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      50 µg

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


    微信号:shjkchem

    活动:消费积分可换充值卡!

    V5介绍:

    货 号:AS15 3027

    中文名称:鼠抗V5标签抗体

    英文名称:V5

    应用:west blot

    规格:50 µg

    价格:3975元

    V5简介:

    PRODUCT INFORMATION
    background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

    immunogen

    KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

    host

    mouse

    clonality

    monoclonal

    clone IgG1 clone V5.E10
    format

    lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide

    quantity 50 µg
    reconstitution For reconstitution add 50 µl of sterile water.
    storage

    Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipitation (IP), immunohistochemistry (IHC)

    related products

    AS15 3031 | V5 (mouse antibody)

    AS15 2964 | V5, ALP conjugated (rabbit antibody)

    AS15 2965 | V5, Biotin conjugated (rabbit antibody)


    AS15 2966 | V5, DyLight® 488 conjugated (rabbit antibody)

    AS15 2967 | V5, DyLight® 550 conjugated (rabbit antibody)

    AS15 2968 | V5, DyLight® 650 conjugated (rabbit antibody)


    AS15 2969 | V5, SureLight® R-Phycoerythrin conjugated (rabbit antibody)


    other tag antibodies
    additional information

    exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

    APPLICATION INFORMATION
    recommended dilution

    1:1000-3000 with ECL (WB), 1:500-2000 (IHC)

    confirmed reactivity

    V5-tagged fusion proteins

    not reactive in

    no confirmed exceptions from predicted reactivity are currently known

    additional information Antibody is purified on Protein A.

    For western blot incubate antibody for one hour at room temperature.
    selected references

    to be added when available antibody available in October 2015

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    图标文献和实验
    相关实验
    • Annexin V 凋亡试剂盒常见问题

      Q:Annexin V 凋亡检测试剂盒的原理是什么? A:Annexin V 是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸 PS 有高度亲和力,可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合,将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,核染料 PI 或 7-AAD

    • Partial proteolysis with Staph. aureus V8 protease

      . If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution.   Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125

    • PCR of the V Region

      To engineer or manipulate antibody or Fv-based molecules, isolation of the V region of the antibodies is necessary. A number of strategies can be adopted for amplification; one such approach is to use subgroup-specific oligonucleotides

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