产品封面图

pET-41b(+)载体

收藏
  • ¥800
  • ZYbscience
  • 中国/美国
  • ZY70557-3
  • 2025年07月15日
    avatar
    上海泽叶生物科技有限公司
    11 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pET-41b(+)

    • 库存：

      20

    • 供应商：

      泽叶生物

    pET41b载体基本信息

    出品公司:
    别名: pET41b, pET 41b
    质粒类型: 大肠杆菌蛋白表达
    表达水平: 高
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 5932 bp
    5' 测序引物: T7或pGEX-5‘
    5' 测序引物序列: T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
    载体标签: N-GST, N-His, N-Thrombin
    载体抗性: Kanamycin
    表达宿主菌: BL21(DE3) 、 Rosetta2(DE3)、C43(DE3)
    备注:
    Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; PshAI blunt cloning site; a,b,c vary by MCS
    产品目录号: ZY70557-3
    稳定性: 瞬时表达 Transient
    组成型: 组成型 Constitutive
    病毒/非病毒: 非病毒

    pET41b载体质粒图谱和多克隆位点信息

    pet41b载体图谱
    pet41b多克隆位点
    pet41b载体克隆方法说明

    Feature Name Start End
    T7_terminator 120 1
    T7_Terminal_primer 69 87
    EK 278 264
    S15 353 309
    6xHIS 413 396
    GST (variant) 1094 444
    T7_transl_en_RBS 1119 1103
    lacO 1164 1137
    T7_promoter 1182 1164
    tet (300 - 563) 1218 1481
    pBRrevBam_primer 1289 1270
    lacI 1564 2655
    ROP 3227 3418
    pGEX_3_primer 3434 3412
    pBR322_origin 4452 3833
    KanR2 4558 5373
    ORF Start End
    ORF frame 3 1094 147
    ORF frame 3 1095 1823
    ORF frame 1 1696 2655
    ORF frame 1 4558 5373
    Enzyme Name Cut
    XhoI 174
    NotI 182
    EagI 182
    HindIII 189
    SalI 195
    SacI 206
    PstI 214
    AscI 216
    StuI 226
    EcoRI 235
    BamHI 241
    EcoRV 251
    NcoI 254
    AgeI 291
    KpnI 298
    BglII 301
    SacII 392
    SpeI 423
    MscI 886
    NdeI 1093
    XbaI 1131
    ApaI 2134
    HpaI 2429
    NruI 4646
    SmaI 4863
    XmaI 4861

    pET41b载体简介

    The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3).Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.

    pET41b载体序列

    LOCUS pET-41 b(+) 5932 bp DNA Circular SYN
    DEFINITION pET-41 b(+)
    ACCESSION
    KEYWORDS
    SOURCE
    ORGANISM other sequences; artificial sequences; vectors.
    COMMENT This file is created by Vector NTI
    http://www.biofeng.com/
    COMMENT ORIGDB|GenBank
    COMMENT VNTAUTHORNAME|biofeng.com|
    COMMENT VNTOAUTHORAD1|Novagen Inc.|
    FEATURES Location/Qualifiers
    source 1..5932
    /organism="pET-41 b(+)"
    /mol_type="other DNA"
    terminator complement(1..120)
    /label="T7_terminator"
    misc_feature 69..87
    /label="T7_Terminal_primer"
    CDS complement(147..1094)
    /label="ORF frame 3"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
    misc_feature complement(264..278)
    /label="EK"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
    misc_feature complement(309..353)
    /label="S15"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
    misc_feature complement(396..413)
    /label="6xHIS"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
    gene complement(444..1094)
    /label="GST (variant)"
    /gene="GST (variant)"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMDIGDPNSVQALARLQASSVDKLAAALEHHHHHHHH*"
    CDS 1095..1823
    /label="ORF frame 3"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1103..1119)
    /label="T7_transl_en_RBS"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1137..1164)
    /label="lacO"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    promoter complement(1164..1182)
    /label="T7_promoter"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    gene 1218..1481
    /label="tet (300 - 563)"
    /gene="tet (300 - 563)"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1270..1289)
    /label="pBRrevBam_primer"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature 1564..2655
    /label="lacI"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    CDS 1696..2655
    /label="ORF frame 1"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature 3227..3418
    /label="ROP"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature complement(3412..3434)
    /label="pGEX_3_primer"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    rep_origin complement(3833..4452)
    /label="pBR322_origin"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 4558..5373
    /label="KanR2"
    /gene="KanR2"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    CDS 4558..5373
    /label="ORF frame 1"
    /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
    GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
    GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
    VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
    RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
    ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
    61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
    121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
    181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
    241 ggatccccga tatccatggg actcttgtcg tcgtcatcac cggagccacc accggtaccc
    301 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca
    361 attgcagtac taccgcgtgg caccagaccc gcggagtgat ggtgatggtg atgaccagaa
    421 ccactagttg aaccatccga ttttggagga tggtcgccac caccaaacgt ggcttgccag
    481 ccctgcaaag gccatgctat atacttgctg gatttcaagt acttatcaat ttgtgggata
    541 gcttcaatac gttttttaaa acaaactaat tttgggaacg catccaggca cattgggtcc
    601 atgtataaaa caacatcaag agcgtcatac aacatgaagt caggatgggt tacatgatca
    661 ccatttaaat atgttttatg acataaacga tcttcgaaca ttttcagcat ttcaggtagc
    721 ttgctaagaa aatcaacttt gagagtttca aagtctttac tatatgcaat tctcgaaaca
    781 ccgtatctaa tatccaaaac cgctccttca agcattgaaa tctctgcacg ctcttttgga
    841 caaccaccca acatgttgtg cttgtcagct atataacgta tgatggccat agactgtgtt
    901 aatttaacat caccatcaat ataataagga agattgggaa actccaaacc caattcaaac
    961 tttttgtttc gccatttatc accttcatcg cgctcataca aatgctcttc atatttttct
    1021 tcaagatatt ccaaaagaag tcgagtgggt tgcacaaggc ccttaatttt ccaataacct
    1081 agtatagggg acatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
    1141 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
    1201 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
    1261 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
    1321 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
    1381 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
    1441 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc
    1501 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
    1561 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
    1621 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
    1681 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
    1741 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
    1801 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
    1861 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
    1921 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
    1981 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
    2041 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
    2101 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
    2161 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
    2221 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
    2281 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
    2341 attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata cgacgatacc
    2401 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
    2461 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
    2521 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
    2581 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    2641 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg
    2701 atctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
    2761 catgactagc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
    2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
    2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
    2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
    3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
    3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
    3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
    3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
    3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
    3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
    3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
    3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
    3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验

    • 用于蛋白质表达和纯化的pET-32α（+）载体的构建

      表达载体的构建是生物技术和所需蛋白质产生的基本工具，本文总结了pET-32α（+）载体技术的构建，该技术通常用于研究实验室。该方法包括获得用于构建载体的外源DNA片段，将DNA片段亚克隆到pET-32α（+）表达载体中，在大肠杆菌 BL21（DE3）中进行蛋白质表达以及在大肠杆菌中在天然条件下进行蛋白质纯化。裂解液。介绍作为分子生物学实验室中非常有用的实践，重组DNA技术（或基因克隆）是指将DNA片段从一种生物体转移到自我复制的遗传元件如表达载体。然后插入的DNA可以在外源宿主细胞中繁殖

    • IPTG诱导pET载体目的蛋白表达的原因分析

      相关专题 1)噬菌体DE3溶源化的菌株如BL21(DE3)是最常用的表达菌株，噬菌体DE3是λ噬菌体的衍生株，构建好的表达载体 可以直接转入表达菌株中，诱导蛋白的表达方式与lac启动子一样都是IPTG诱导。 2)表达菌株BL21(DE3)上带有lacI基因，T7 RNA 聚合酶编码基因以及lacUV5启动子，lacUV5启动子可以启动T7 RNA 聚合酶的表达; 3)表达质粒 如pET-28质粒带有T7启动子和编码阻遏

    • 如何阅读质粒图谱

      （ DE3 ）表达的活性蛋白比其它宿主菌高 10 倍以上。 Origami 宿主菌与氨苄抗性质粒相容，可用于 pET-32 载体，硫氧还蛋白标签能够进一步增强在胞浆内形成二硫键。 TrxB 和 gor 突变可分别用卡那霉素和四环素选择，因此该菌株建议用于带氨苄抗性标记 bla 的 pET 质粒。 Origami B 宿主菌来源于 BL21 lacZY 突变株，还带有与原始 Origami 菌株相同的 TrxB / gor 突变。 Origami B 菌株集 BL21 、 Tuner

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    载体pET-41b
    ¥800
    上海沪震实业有限公司
    2025年07月16日询价
    pET-41b(+)载体
    ¥1000
    上海再康生物科技有限公司
    2025年11月20日询价
    pET-41b(+)载体
    ¥1000
    上海信裕生物科技有限公司
    2025年07月08日询价
    pET-41a(+)载体
    ¥1100
    上海泽叶生物科技有限公司
    2025年07月16日询价
    pET-41c(+)载体
    ￥1000
    上海盖宁生物科技有限公司
    2026年03月20日询价
    pET-41b(+)载体
    ¥800