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- cellvis
- P384-1.5H-N
- 2026年04月26日
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- 详细信息
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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
杭州欣友生物技术有限公司
- 现货状态：
现货
- 规格：
1个/包，20个/箱
玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验，如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄，透光性能好， 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片，产品采用无细胞毒性的医用胶水粘合，适用于激光共聚焦等需要高分辨率的细胞显微实验，并且能够耐受长期的细胞培养。
384孔玻底板使用专门的模具生产，产品均一，使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯和医用级的黑色母为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产，保证洁净度。
玻底培养皿和玻底培养板使用方法(以35mm皿，10mm孔为例)
- 预平衡：在玻底培养皿加入3ml培养液，在培养箱中放置15分钟。
- 加细胞：吸去培养液，在底孔中加入500ul含细胞的培养液。在培养箱中放置2小时，让细胞沉降贴壁。
- 加培养液：小心加入2-3ml不含细胞的培养液。该步骤用于为细胞提供足够的培养液，同时减少由于水份挥发带来的渗透压的变化。
*根据实验的要求，可以合并步骤2和3，在预平衡后直接加入2-3毫升含细胞的培养液。
www.sybio.com
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- 作者
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文献和实验- Initial niche condition determines the aging speed and regenerative activity of quiescent cells
Qi Liu, et al., bioRxiv - Cell Biology 2022
Quote: ... For coating 384well microscope plate (Cellvis, Cat# P384-1.5H-N), fill each well with 20μl Concanavalin A water solution (2mg/ml ... - Specific viral RNA drives the SARS CoV-2 nucleocapsid to phase separate
Christiane Iserman, et al., bioRxiv - Biochemistry 2020
Quote: ... The mix was incubated in 384-well plates (Cellvis P384-1.5H-N) for 16 hours at 37°C unless indicated otherwise ... - Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures
Christine A. Roden, et al., bioRxiv - Molecular Biology 2021
Quote: The mix was incubated in 384-well plates (Cellvis P384-1.5H-N) at 25 ... - Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures
Christine A. Roden, et al., bioRxiv - Molecular Biology 2021
Quote: ... The mix was incubated in 384-well plates (Cellvis P384-1.5H-N) for 1-20 hours at 37°C unless indicated otherwise ... - Condensation of LINE-1 is required for retrotransposition
Srinjoy Sil, Jef D Boeke, Liam J Holt, bioRxiv - Cell Biology 2022
Quote: ... the wells of a 384-well glass-bottom plate (Cellvis, product number P384-1.5H-N) were blocked as described in (Keenen ... - Analysis of novel hyperosmotic shock response suggests "beads in liquid" cytosol structure
Alexander I Alexandrov, et al., bioRxiv - Cell Biology 2019
Quote: ... washed with YP-Gly and moved to 384 glass-bottom plates (CellVis, P384-1.5H-N, USA). Each sample was placed into two adjacent wells and after loading ... - DNA Origami Presenting the Receptor Binding Domain of SARS-CoV-2 Elicit Robust Protective Immune Response
Esra Oktay, et al., bioRxiv - Bioengineering 2022
Quote: ... Greiner 384-well polystyrene flat bottom microplate was purchased from Cellvis (cat no: P384-1.5H-N). PageBlue Protein Staining Solution (cat ... - FMRP phosphorylation modulates neuronal translation through YTHDF1
Zhongyu Zou, et al., bioRxiv - Molecular Biology 2022
Quote: ... the mixtures were prepared in vitro on a 384-well plate with coverslip bottom (Cellvis, P384-1.5H-N). Image acquirement and processing were conducted similarly. - Uncovering molecular grammars of intrinsically disordered regions that organize nucleolar fibrillar centers
Matthew R. King, et al., bioRxiv - Biophysics 2022
Quote: ... All dilutions were performed directly in individual wells in a glass-bottomed 384-well plate (Cellvis-P384-1.5H-N). Immediately upon dilution these mixtures were mixed by pipetting up and down three times ... - Phase separation of RNA-binding protein promotes polymerase engagement and transcription
Wen Shao, et al., bioRxiv - Biochemistry 2021
Quote: In vitro droplet formation was performed in tubes and visualized in 384-well glass-bottom plates (In Vitro Scientific, P384-1.5H-N). Proteins were diluted to indicated concentrations in a buffer containing 20 mM Tris pH 7.5 and 150 mM NaCl supplemented with or without 10% dextran.
Cited publications before 2019 (3)
-
Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation
Shuai Zhao, et al., Cell Research, volume 29, pages54–66 (2019)
Quote: "Equal volume of nucleosome array and ADCP1 were mixed in a 384 well glass bottom plate (Cellvis, P384-1.5H-N)." -
Multimodal on-axis platform for all-optical electrophysiology with near-infrared probes in human stem-cell-derived cardiomyocytes
A Klimas,, BioRxiv, February 21, 2018
Quote: "human iPSC-derived cardiomyocytes (iCell Cardiomyocytes2 ™, Cellular Dynamics International (CDI), Madison, WI) were thawed per the manufacturer's instructions and plated on fibronectin coated wells in 384-well glass-bottom plates (P384-1.5HN, Cellvis, Mountain View)" -
Label-free cell-based assay with spectral-domain optical coherence phase microscopy
Suho Ryu et al., Journal of Biomedical Optics, vol19, issue 4, 2014
Quote: "The 100 μl of the sample was loaded on glass bottom multiwell plates (In Vitro Scientific, Sunnyvale, California, P384-1.5HN) and incubated for 24 h prior to experiment."
孔板甚至同一孔内多点拍摄，无需手动调整细胞培养板，非常方便，可以尽量减少细胞在培养箱外待的时间。 此外，本研究中使用的聚苯乙烯的细胞培养板与常规的共聚焦玻底皿不同，这种差异使得在成像过程中不但工作距离有所改变，还需要额外的光学校准。 Evident 全新的 X Line 物镜，依靠超薄透镜打磨技术和超薄透镜组装技术，使图像平场性、数值孔径和色差校正范围三大核心参数同步得到提升，还提供盖玻片厚度、工作距离、浸油折射率等多种校正，为实验提供更专业可靠的支持。 参考文献： [1] M
细胞培养板的选择：细胞培养板依底部形状的不同可分为平底和圆底（U型和V型）；培养孔的孔数有6、12、24、48、96、384孔等；根据材质的不同有Terasaki板和普通细胞培养板。具体选择时根据培养细胞的类型、所需培养体积及不同的实验目的而定。 平底和圆底（U型和V型）培养板的区别和选择： 贴壁细胞一般用平底培养板 悬浮型细胞的培养一般用V型 U型培养板亦多用于培养悬浮型细胞 不同型状的板子自然有不同用途。平底的什么类型的细胞都可用﹐但当细胞
Life Technologies根据不同细胞类型设计生产了硬度各异的Softwell透明水凝胶，并已经将这些水凝胶铺设在我们常用细胞培养板、皿等用材的表面，用户可以根据所培养细胞的体内环境选择相应硬度的水凝胶培养板或培养皿，十分方便。 Matrigen Life Technologies专一致力于重塑细胞培养之景象：你只需简单的改变培养用品即可让你的细胞培养条件更接近于体内真实状况。Matrigen Life Technologies生产的Softwell水凝胶产品主要包括如下三种
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