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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-80º C
- 保质期：
一年
- 英文名：
Endura™ ElectroCompetent Cells
- 库存：
大量
- 供应商：
中北林格
- CAS号：
8888-88-8
- 规格：
24 rxns (DUOs)
Endura™ Competent Cells
产品说明书
- Clone unstable sequences with reliable results
- Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain
- Available as chemically competent or electrocompetent cells
- Your choice of two packaging formats
- High efficiency: over 1 × 107 cfu/µg (chem) or 1 × 1010 cfu/µg (electro)
- Ideal for CRISPR GeCKO Libraries
- Excellent value
Conserve clones that contain unstable sequences!
Maintain unstable DNA. Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Excellent value and efficiency! Switch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.
Chemical or Electrocompetent. Whichever method of transformation you prefer, Lucigen can give you better efficiency and/or better prices.
Figure 1. Cost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.
Figure 2. Transformation efficiencies of electrocompetent clone-stabilizing strains.
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Ordering
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Specifications
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Manuals
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Resources
| Product Description | Size | Cat. No. | |
| Endura™ Chemically Competent Cells | 4 rxns (DUO) | 60240-0 | |
| 12 rxns (DUO) | 60240-1 | ||
| 24 rxns (DUO) | 60240-2 | ||
| 12 rxns (SOLO) | 60241-1 | ||
| 24 rxns (SOLO) | 60241-2 | ||
| Endura™ ElectroCompetent Cells | 4 rxns (DUO) | 60242-0 | |
| 12 rxns (DUO) | 60242-1 | ||
| 24 rxns (DUO) | 60242-2 | ||
Endura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.
NOTE: Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium. The Zhang lab protocol (available from Addgene) recommends ~2 mL of Recovery Medium per transformation. Lucigen Endura kits provide 1 mL of Recovery Medium per transformation.
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Blood Cells: A Practical Guide
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Belcher/Knight: Electrocompetent Cells
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Transformation of Electrocompetent E. coli with Blue/White selection
to determine the efficiency of competent cells: Efficiency (Transformants/µg) = colonies/plate x (Bacterial dilution factor) x 20,000 Highly competent cells should have ~50 colonies on the plate with a 1/10 dilution of the control DNA
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