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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
NCI-H358
- 库存:
大量
- 供应商:
中乔新舟
- 细胞类型:
细胞系
- 品系:
human
- 组织来源:
human
- 相关疾病:
否
- 物种来源:
human
- 免疫类型:
否
- 细胞形态:
咨询销售
- 器官来源:
human
- 运输方式:
T25瓶运输
- 年限:
5-10年
- 生长状态:
贴壁生长
- 规格:
5 x 10^5 cells/vial
|
产品名称 |
NCI-H358人非小细胞肺癌细胞 |
|
货号 |
ZQ0384 |
|
产品介绍 |
NCI-H358,也被称为H-358或NCIH358,是一种上皮样细胞系,来源于一名非小细胞肺癌(NSCLC)亚型细支气管肺泡癌患者。超微结构研究表明细胞质中有Clara细胞的特征结构 细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。 不表达SP-B和SP-C。 他们在软琼脂中的克隆形成效率为0.83%。NCI-H358细胞在以非小细胞肺癌为重点的癌症研究中尤其重要,特别是在探索肺腺癌的生物学和治疗方面。 |
|
种属 |
人 |
|
性别/年龄 |
男 |
|
组织 |
肺/支气管;来源于转移部位:肺泡 |
|
疾病 |
细支气管肺泡癌;非小细胞肺癌 |
|
细胞类型 |
肿瘤细胞 |
|
形态学 |
上皮细胞 |
|
生长方式 |
贴壁 |
|
倍增时间 |
大约38~76小时 |
|
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
|
推荐完全培养基货号 |
ZM0384 |
|
生物安全等级 |
BSL-1 |
|
STR位点信息 |
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 TH01: 6 TPOX: 8,9 vWA: 17 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
|
抗原表达/受体表达 |
*** |
|
基因表达 |
*** |
|
保藏机构 |
ATCC; CRL-5807 ECACC; 95111733 |
|
供应限制 |
仅供科研使用 |
上海中乔新舟生物科技有限公司成立于2011年,历经十多年发展,主要专注于细胞生物学产品的研究和开发,专注于为药企、各类科研机构及CRO企业提供符合标准规范的细胞培养服务、细胞培养基、细胞检测试剂盒、细胞培养试剂,胎牛血清和细胞生物学技术服务等。
公司一直致力于为高等院校、研究机构、医院、CRO及CDMO企业提供细胞培养完整解决方案,这些产品旨在满足细胞培养的多样需求,确保实验和研究的有效进行。引用中乔新舟(ZQXZBIO)产品和服务的文献超数千篇。

产品服务
细胞资源:原代细胞、细胞株、干细胞、示踪细胞、耐药株细胞、永生化细胞等基因工程细胞。
试剂产品:胎牛血清、完全培养基(适用于原代细胞及细胞株)、无血清培养基、基础培养基、细胞转染试剂、重组因子、胰酶和双抗等等细胞培养所有实验相关产品。
技术服务:稳转株构建、原代细胞分离、特殊培养基定制服务、细胞检测等。

目前产品已经畅销国内30多个省市,与客户建立长期的合作伙伴关系,共同实现成功。全体员工将不懈努力,继续为科研人员提供优良的产品和服务,致力成为全球细胞培养领域的参与者。

企业愿景
致力于成为国内细胞培养基产业的佼佼者,生物医药领域上游原材料的优良提供商。
企业使命
成长为专业细胞系及原代细胞培养供应商、专业细胞培养基及培养试剂生产商。
企业荣誉


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文献和实验论文标题: NEDD4L-induced ubiquitination mediating UBE2T degradation inhibits progression of lung adenocarcinoma via PI3K-AKT signaling
DOI: 10.1186/s12935-021-02341-9
发表时间: 2021-11-27
期刊: Cancer Cell International
影响因子: 5.722
货号: ZQ0384
产品名称: H358 cells
论文标题: LncRNA PCBP1-AS1 correlated with the functional states of cancer cells and inhibited lung adenocarcinoma metastasis by suppressing the EMT progression
DOI: 10.1093/carcin/bgab047
发表时间: 2021-07-16
期刊: CARCINOGENESIS
影响因子: 4.944
货号: ZQ0384
产品名称: H358 cells
论文标题: High expression of 14‑3‑3ơ indicates poor prognosis and progression of lung adenocarcinoma
DOI: 10.3892/ol.2022.13323
发表时间: 2022-05-12
期刊: Oncology Letters
影响因子: 2.967
货号: ZQ0384
产品名称: NCI-H358 cells
论文标题: Loading MicroRNA-376c in Extracellular Vesicles Inhibits Properties of Non-Small Cell Lung Cancer Cells by Targeting YTHDF1:
DOI: 10.1177/1533033820977525
发表时间: 2020-12-07
期刊: TECHNOLOGY IN CANCER RESEARCH & TREATMENT
影响因子: 2.074
货号: ZQ0384
产品名称: NCI-H358 cells
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都是贴壁细胞,在消化传代过程中,步骤如下:倒尽旧的培养液->用无血清的培养基清洗一两次->加入一定量的胰酶,置于37度培养箱中5--10分钟,使细胞悬浮->显微镜下观察,待细胞大部分变圆时,回到超静台->加入一定量的含血清的新培养液,以终止胰酶作用->反复吹打细胞->再置显微镜下观察,直到细胞全部悬浮起来->吸出一部分加入新的培养瓶中->最后再补充加入一定量新的培养液。注意: 1、吹细胞时尽量多吹边角儿,此处细胞生长的多。2、吸出细胞前要混匀,可以剧烈震荡培养瓶。3、我们用的是DMEM
CD44变异体V6、V7/8在非小细胞肺癌中的表达及临床意义
CD44变异体V 6 、V 7/8 在非小细胞肺癌中的表达及临床意义 第三军医大学学报2000年第22卷第7期 孙焰 陈幸华 罗平 贺光友 摘 要 : 目的 探讨CD44 v 6 、V 7/8 与非小细胞肺癌(Non-small cell lung cancer,NSCLC)患者的淋巴结转移及预后的关系。 方法 采用免疫组化方法观察CD44 v 6 、V 7/8 在76例原发性非小细胞肺癌
每年的 11 月 17 日被定为「国际肺癌宣传日」,旨在呼吁世界各国政府重视肺癌的预防,提高人们对肺癌的防治意识,普及肺癌的规范化诊疗知识。肺癌是我国及世界范围内发病率和死亡率较高的恶性肿瘤之一,一般分非小细胞肺癌(non small cell lung cancer, NSCLC)和小细胞肺癌(small cell lung cancer,SCLC)两种亚型,其中非小细胞肺癌约占肺癌的 80~85%[1]。 图 1. 2015 年中国肿瘤发病率统计 目前,已发现的肺癌驱动基因已达数十
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