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文献和实验The determination of the protein content of urokinase-type plasminogen activator (uPA) and its inhibitor, PAI-1, in breast cancer tissue extracts is used clinically to identify patients at risk to experience disease recurrence (metastasis
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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hedgehog fused to murine and rat IgG1 hinge and FcS, α-MF, active508ThrombomodulinS[274]Tissue factor extracellular domainS, 10 mg/L, PHO1[275]Tissue kallikreinS, 30 mg/L, α-MF[276, 277]Tissue-type plasminogen activator kringle 2 domainS, 170 mg/L, α-MF[278
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